PMID- 24798339
OWN - NLM
STAT- MEDLINE
DCOM- 20141006
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 25
DP  - 2014 Jun 20
TI  - Distinct roles for release factor 1 and release factor 2 in translational quality 
      control.
PG  - 17589-96
LID - 10.1074/jbc.M114.564989 [doi]
AB  - In bacteria, stop codons are recognized by two similar class 1 release factors, 
      release factor 1 (RF1) and release factor 2 (RF2). Normally, during termination, 
      the class 2 release factor 3 (RF3), a GTPase, functions downstream of peptide 
      release where it accelerates the dissociation of RF1/RF2 prior to ribosome 
      recycling. In addition to their canonical function in termination, both classes 
      of release factor are also involved in a post peptidyl transfer quality control 
      (post PT QC) mechanism where the termination factors recognize mismatched (i.e. 
      error-containing) ribosome complexes and promote premature termination. Here, 
      using a well defined in vitro system, we explored the role of release factors in 
      canonical termination and post PT QC. As reported previously, during canonical 
      termination, RF1 and RF2 recognize stop codons in a similar manner, and RF3 
      accelerates their rate of dissociation. During post PT QC, only RF2 (and not RF1) 
      effectively binds to mismatched ribosome complexes; and whereas the addition of 
      RF3 to RF2 increased its rate of release on mismatched complexes, the addition of 
      RF3 to RF1 inhibited its rate of release but increased the rate of peptidyl-tRNA 
      dissociation. Our data strongly suggest that RF2, in addition to its primary role 
      in peptide release, functions as the principle factor for post PT QC.
CI  - (c) 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Petropoulos, Alexandros D
AU  - Petropoulos AD
AD  - From the Howard Hughes Medical Institute, Department of Molecular Biology and 
      Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 
      21205 and.
FAU - McDonald, Megan E
AU  - McDonald ME
AD  - From the Howard Hughes Medical Institute, Department of Molecular Biology and 
      Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 
      21205 and.
FAU - Green, Rachel
AU  - Green R
AD  - From the Howard Hughes Medical Institute, Department of Molecular Biology and 
      Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 
      21205 and.
FAU - Zaher, Hani S
AU  - Zaher HS
AD  - the Department of Biology, Washington University in St. Louis, St. Louis, 
      Missouri 63130 hzaher@wustl.edu.
LA  - eng
GR  - R00 GM094210/GM/NIGMS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01 GM059425/GM/NIGMS NIH HHS/United States
GR  - R00GM094210/GM/NIGMS NIH HHS/United States
GR  - R01GM059425-06A1/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140505
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Peptide Termination Factors)
RN  - 0 (prfA protein, E coli)
RN  - 0 (prfB protein, E coli)
SB  - IM
MH  - Escherichia coli/genetics/*metabolism
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Peptide Chain Termination, Translational/*physiology
MH  - Peptide Termination Factors/genetics/*metabolism
MH  - Ribosomes/genetics/*metabolism
PMC - PMC4067194
OTO - NOTNLM
OT  - Protein Synthesis
OT  - Ribosome
OT  - Translation
OT  - Translation Control
OT  - Translation Release Factor
EDAT- 2014/05/07 06:00
MHDA- 2014/10/07 06:00
PMCR- 2015/06/20
CRDT- 2014/05/07 06:00
PHST- 2014/05/07 06:00 [entrez]
PHST- 2014/05/07 06:00 [pubmed]
PHST- 2014/10/07 06:00 [medline]
PHST- 2015/06/20 00:00 [pmc-release]
AID - S0021-9258(20)40600-3 [pii]
AID - M114.564989 [pii]
AID - 10.1074/jbc.M114.564989 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Jun 20;289(25):17589-96. doi: 10.1074/jbc.M114.564989. Epub 
      2014 May 5.