PMID- 24799764
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20220318
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2014
DP  - 2014
TI  - MicroRNA profiling of activated and tolerogenic human dendritic cells.
PG  - 259689
LID - 10.1155/2014/259689 [doi]
LID - 259689
AB  - Dendritic cells (DCs) belong to the immune system and are particularly studied 
      for their potential to direct either an activated or tolerogenic immune response. 
      The roles of microRNAs (miRNAs) in posttranscriptional gene expression regulation 
      are being increasingly investigated. This study's aim is to evaluate the miRNAs' 
      expression changes in prepared human immature (iDCs), activated (aDCs), and 
      tolerogenic dendritic cells (tDCs). The dendritic cells were prepared using 
      GM-CSF and IL-4 (iDC) and subsequently maturated by adding LPS and IFN-gamma (aDC) or 
      IL-10 and TGF-beta (tDC). Surface markers, cytokine profiles, and miRNA profiles 
      were evaluated in iDC, tDC, and aDC at 6 h and 24 h of maturation. We identified 
      4 miRNAs (miR-7, miR-9, miR-155 and miR-182), which were consistently 
      overexpressed in aDC after 6 h and 24 h of maturation and 3 miRNAs (miR-17, 
      miR-133b, and miR-203) and miR-23b cluster solely expressed in tDC. We found 5 
      miRNAs (miR-10a, miR-203, miR-210, miR-30a, and miR-449b) upregulated and 3 
      miRNAs downregulated (miR-134, miR-145, and miR-149) in both tDC and aDC. These 
      results indicate that miRNAs are specifically modulated in human DC types. This 
      work may contribute to identifying specific modulating miRNAs for aDC and tDC, 
      which could in the future serve as therapeutic targets in the treatment of cancer 
      and autoimmune diseases.
FAU - Stumpfova, Zuzana
AU  - Stumpfova Z
AD  - Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 
      62500 Brno, Czech Republic.
FAU - Hezova, Renata
AU  - Hezova R
AD  - CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk 
      Memorial Cancer Institute, Zluty Kopec 7, 65653 Brno, Czech Republic.
FAU - Meli, Albano Carlo
AU  - Meli AC
AD  - Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500 
      Brno, Czech Republic ; INSERM U1046, University of Montpellier I and II, 371 
      avenue du Doyen G. Giraud, 34295 Montpellier, France.
FAU - Slaby, Ondrej
AU  - Slaby O
AD  - CEITEC, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic ; Masaryk 
      Memorial Cancer Institute, Zluty Kopec 7, 65653 Brno, Czech Republic.
FAU - Michalek, Jaroslav
AU  - Michalek J
AD  - Department of Pharmacology, Faculty of Medicine, Masaryk University, Kamenice 5, 
      62500 Brno, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140401
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MIRN10 microRNA, human)
RN  - 0 (MIRN133 microRNA, human)
RN  - 0 (MIRN17 microRNA, human)
RN  - 0 (MIRN203 microRNA, human)
RN  - 0 (MIRN210 microRNA, human)
RN  - 0 (MIRN30b microRNA, human)
RN  - 0 (MIRN449 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Interleukin-10/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - MicroRNAs/*genetics
MH  - Transforming Growth Factor beta/pharmacology
PMC - PMC3995309
EDAT- 2014/05/07 06:00
MHDA- 2014/12/19 06:00
PMCR- 2014/04/01
CRDT- 2014/05/07 06:00
PHST- 2013/11/15 00:00 [received]
PHST- 2014/01/19 00:00 [revised]
PHST- 2014/02/21 00:00 [accepted]
PHST- 2014/05/07 06:00 [entrez]
PHST- 2014/05/07 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1155/2014/259689 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2014;2014:259689. doi: 10.1155/2014/259689. Epub 2014 Apr 1.