PMID- 24800957
OWN - NLM
STAT- MEDLINE
DCOM- 20150622
LR  - 20141023
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 38
IP  - 10
DP  - 2014 Oct
TI  - Polyamines cause elevation of steroid 5alpha-reductase mRNA levels by suppressing 
      mRNA degradation in C6 glioma cells.
PG  - 1132-7
LID - 10.1002/cbin.10309 [doi]
AB  - Polyamines are widely distributed in living organisms, and considered to play a 
      potential role in various cellular processes. The effects of polyamines on gene 
      expression as well as cell proliferation have been suggested to be closely 
      associated with the physiological and pathological functions. However, it seems 
      necessary to investigate their potential roles in the regulation of cellular 
      metabolism and functions. Previously, glial cells have been suggested to be 
      involved in the protection and preservation of neuronal functions, probably 
      through the production of neurotrophic factors in the brain. On the other hand, 
      neuroactive 5alpha-reduced steroids promote glial cell differentiation, resulting in 
      enhancement of their ability to produce brain-derived neurotrophic factor (BDNF). 
      Based on these findings, polyamines are assumed to stimulate the expression of 
      the gene encoding steroid 5alpha-reductase (5alpha-R), which can induce the production of 
      neuroactive 5alpha-reduced steroids in glial cells. The effects of polyamines on 5alpha-R 
      mRNA levels in C6 glioma cells were examined as a model experiment. In 
      consequence, spermine (SPM) and spermidine (SPD), but not putrescine (PUT), have 
      been shown to elevate 5alpha-R mRNA levels without activating the 5alpha-R promoter. 
      Furthermore, SPM increased 5alpha-R mRNA levels under the conditions in which the 
      mRNA biosynthesis was inhibited. Therefore, it can be speculated that polyamines 
      increase 5alpha-R mRNA levels as a consequence of suppressing the degradation of 
      mRNA.
CI  - (c) 2014 International Federation for Cell Biology.
FAU - Morita, Kyoji
AU  - Morita K
AD  - Laboratory of Neuropharmacology, Department of Nursing, Shikoku University School 
      of Health Sciences, Tokushima, 771-1192, Japan.
FAU - Lee, Mi-Sook
AU  - Lee MS
FAU - Her, Song
AU  - Her S
FAU - Nishibori, Naoyoshi
AU  - Nishibori N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140529
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Polyamines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sp1 Transcription Factor)
RN  - EC 1.3.99.5 (3-Oxo-5-alpha-Steroid 4-Dehydrogenase)
SB  - IM
MH  - 3-Oxo-5-alpha-Steroid 4-Dehydrogenase/genetics/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Line, Tumor
MH  - Glioma/metabolism/pathology
MH  - Oligonucleotides, Antisense/metabolism
MH  - Polyamines/*pharmacology
MH  - Promoter Regions, Genetic
MH  - RNA Interference
MH  - RNA Stability/*drug effects
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Sp1 Transcription Factor/antagonists & inhibitors/genetics/metabolism
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - actinomycin D
OT  - mRNA stabilization
OT  - neuroactive steroids
OT  - promoter activity
EDAT- 2014/05/08 06:00
MHDA- 2015/06/24 06:00
CRDT- 2014/05/08 06:00
PHST- 2013/10/27 00:00 [received]
PHST- 2014/04/14 00:00 [accepted]
PHST- 2014/05/08 06:00 [entrez]
PHST- 2014/05/08 06:00 [pubmed]
PHST- 2015/06/24 06:00 [medline]
AID - 10.1002/cbin.10309 [doi]
PST - ppublish
SO  - Cell Biol Int. 2014 Oct;38(10):1132-7. doi: 10.1002/cbin.10309. Epub 2014 May 29.