PMID- 24801253
OWN - NLM
STAT- MEDLINE
DCOM- 20150121
LR  - 20211021
IS  - 1552-485X (Electronic)
IS  - 1552-4841 (Print)
IS  - 1552-4841 (Linking)
VI  - 165B
IP  - 4
DP  - 2014 Jun
TI  - Altered DNA methylation status of human brain derived neurotrophis factor gene 
      could be useful as biomarker of depression.
PG  - 357-64
LID - 10.1002/ajmg.b.32238 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is involved in the survival, 
      development, and synaptic plasticity of neurons. BDNF is believed to be 
      associated with the pathophysiology of psychiatric disorders. Several studies 
      have suggested the relevance of DNA methylation in its promoter region with 
      depression. Here, we report different methylation statuses in groups with 
      different depressive scores or undergoing different levels of job-stress. DNA 
      samples were extracted from the saliva of 774 Japanese workers, and the 
      methylation status was determined using the Illumina HumanMethylation 450 K 
      Microarray. Depressive symptoms were measured using the Kessler's K6 
      questionnaire. Job-stress scales were assessed via a self-administered 
      questionnaire. Independent DNA pools were formed based on K6 and job-strain 
      scores, and the methylation levels were compared among these pools. The average 
      DNA methylation rate was significantly decreased in the highest K6 score group 
      compared to the lowest group (methylated signals, 14.2% vs. 16.5%, 
      P = 2 . 16 x 10(-198)). This difference remained for the CpG island in the 
      promoter region (10.4% vs. 5.8%, P = 3 . 67 x 10(-133)). Regarding the job-strain 
      score, there was a slight increase in the methylation level of the whole gene in 
      the group with the highest score compared to that with the lowest score; however, 
      these groups showed no difference in the promoter region. Our results revealed 
      significant changes in the DNA methylation status of the complete human BDNF gene 
      in persons with depression compared to normal individuals, especially in the 
      promoter region of exon 1. This indicates that DNA methylation in this gene is a 
      promising biomarker for diagnosing depression.
CI  - (c) 2014 The Authors. American Journal of Medical Genetics Part B: Neuropsychiatric 
      Genetics Published by Wiley Periodicals, Inc.
FAU - Song, Yixuan
AU  - Song Y
AD  - Department of Clinical Research and Informatics, National Center for Global 
      Health and Medicine, Tokyo, Japan.
FAU - Miyaki, Koichi
AU  - Miyaki K
FAU - Suzuki, Tomoko
AU  - Suzuki T
FAU - Sasaki, Yasuharu
AU  - Sasaki Y
FAU - Tsutsumi, Akizumi
AU  - Tsutsumi A
FAU - Kawakami, Norito
AU  - Kawakami N
FAU - Shimazu, Akihito
AU  - Shimazu A
FAU - Takahashi, Masaya
AU  - Takahashi M
FAU - Inoue, Akiomi
AU  - Inoue A
FAU - Kan, Chiemi
AU  - Kan C
FAU - Kurioka, Sumiko
AU  - Kurioka S
FAU - Shimbo, Takuro
AU  - Shimbo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140507
PL  - United States
TA  - Am J Med Genet B Neuropsychiatr Genet
JT  - American journal of medical genetics. Part B, Neuropsychiatric genetics : the 
      official publication of the International Society of Psychiatric Genetics
JID - 101235742
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Adult
MH  - Biomarkers/metabolism
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - CpG Islands/genetics
MH  - DNA Methylation/*genetics
MH  - Depression/*genetics
MH  - Female
MH  - Humans
MH  - Life Style
MH  - Male
MH  - Socioeconomic Factors
MH  - Stress, Psychological/genetics
PMC - PMC4321058
OTO - NOTNLM
OT  - BDNF
OT  - HumanMethylation 450K Microarray
OT  - K6 score
OT  - epigenetic
EDAT- 2014/05/08 06:00
MHDA- 2015/01/22 06:00
CRDT- 2014/05/08 06:00
PHST- 2013/11/04 00:00 [received]
PHST- 2014/04/14 00:00 [accepted]
PHST- 2014/05/08 06:00 [entrez]
PHST- 2014/05/08 06:00 [pubmed]
PHST- 2015/01/22 06:00 [medline]
AID - 10.1002/ajmg.b.32238 [doi]
PST - ppublish
SO  - Am J Med Genet B Neuropsychiatr Genet. 2014 Jun;165B(4):357-64. doi: 
      10.1002/ajmg.b.32238. Epub 2014 May 7.