PMID- 24801397
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20151119
IS  - 1557-8674 (Electronic)
IS  - 1096-2964 (Linking)
VI  - 15
IP  - 3
DP  - 2014 Jun
TI  - Detection of different virus-specific CD8+ T cells after kidney transplantation.
PG  - 274-82
LID - 10.1089/sur.2013.083 [doi]
AB  - BACKGROUND: The early diagnosis of viral reactivation after kidney 
      transplantation (KTX) is an unsolved problem. Survey of virus-specific T-cell 
      responses may identify patients at risk for viral reactivation. We therefore 
      quantified virus-specific CD8+ T-cells to evaluate their potential predictive 
      value for viral reactivation and infection in KTX patients. METHODS: We 
      quantified the virus-specific responses of CD8+ T-cells for CMV, EBV, HPV and HHV 
      in 23 patients undergoing KTX for 6 mo after transplantation. We enumerated 
      T-cells for 36 virus-specific binding peptides and five different human leukocyte 
      antigen (HLA) alleles through the binding of Class I iTAg major 
      histocompatibility complex (MHC) tetramers. The patients' pre-operative serologic 
      status for CMV and CMV-specific CD8+ T-cell numbers were correlated with one 
      another (p=0.0046). RESULTS: Three patients had clinical CMV disease and all 
      three remained or became CMV-tetramer-positive for at least one HLA allele during 
      follow-up. Three of the four patients with viral infections caused by or 
      reactivations of viruses other than CMV were initially negative for CMV-specific 
      CD8+ T-cells but became CMV-positive. Most of the patients who were initially 
      CMV-tetramer positive also had tetramer-positive T-cells specific for 
      Epstein-Barr virus (EBV); human papillomavirus (HPV)-6b, -11, -16, or -18; or 
      human herpesvirus (HHV)-8. All of the patients who developed viral disease other 
      than that caused by CMV remained or became positive for at least one binding 
      peptide that was specific for a virus not directly related to the clinical 
      features of a viral disease. CONCLUSION: Patients who were positive for any virus 
      had a significantly greater risk of developing complications of viral disease 
      during the 6-mo follow-up period in the study (p=0.026), suggesting a general 
      susceptibility to viral reactivation. The evaluation of virus-specific CD8+ 
      T-cells may prospectively help to identify patients at risk for viral 
      reactivation after KTX.
FAU - Mees, Soeren Torge
AU  - Mees ST
AD  - 1 Department of General and Visceral Surgery, University Hospital Muenster , 
      Muenster, Germany .
FAU - Kebschull, Linus
AU  - Kebschull L
FAU - Mardin, Wolf Arif
AU  - Mardin WA
FAU - Senninger, Norbert
AU  - Senninger N
FAU - Suwelack, Barbara
AU  - Suwelack B
FAU - Wolters, Heiner
AU  - Wolters H
FAU - Haier, Joerg
AU  - Haier J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140506
PL  - United States
TA  - Surg Infect (Larchmt)
JT  - Surgical infections
JID - 9815642
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Female
MH  - Herpesviridae/*immunology
MH  - Humans
MH  - *Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Papillomaviridae/*immunology
MH  - Prospective Studies
MH  - Virus Activation/*immunology
MH  - Virus Diseases/*diagnosis/*immunology
MH  - Young Adult
EDAT- 2014/05/08 06:00
MHDA- 2015/02/05 06:00
CRDT- 2014/05/08 06:00
PHST- 2014/05/08 06:00 [entrez]
PHST- 2014/05/08 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
AID - 10.1089/sur.2013.083 [doi]
PST - ppublish
SO  - Surg Infect (Larchmt). 2014 Jun;15(3):274-82. doi: 10.1089/sur.2013.083. Epub 
      2014 May 6.