PMID- 24803493 OWN - NLM STAT- MEDLINE DCOM- 20150819 LR - 20211021 IS - 1552-6844 (Electronic) IS - 1545-9683 (Print) IS - 1545-9683 (Linking) VI - 29 IP - 1 DP - 2015 Jan TI - Either brain-derived neurotrophic factor or neurotrophin-3 only neurotrophin-producing grafts promote locomotor recovery in untrained spinalized cats. PG - 90-100 LID - 10.1177/1545968314532834 [doi] AB - Background. Transplants of cellular grafts expressing a combination of 2 neurotrophic factors, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) have been shown to promote and enhance locomotor recovery in untrained spinalized cats. Based on the time course of recovery and the absence of axonal growth through the transplants, we hypothesized that recovery was due to neurotrophin-mediated plasticity within the existing locomotor circuitry of the lumbar cord. Since BDNF and NT-3 have different effects on axonal sprouting and synaptic connectivity/strengthening, it becomes important to ascertain the contribution of each individual neurotrophins to recovery. Objective. We studied whether BDNF or NT-3 only producing cellular grafts would be equally effective at restoring locomotion in untrained spinal cats. Methods. Rat fibroblasts secreting one of the 2 neurotrophins were grafted into the T12 spinal transection site of adult cats. Four cats in each group (BDNF alone or NT-3 alone) were evaluated. Locomotor recovery was tested on a treadmill at 3 and 5 weeks post-transection/grafting. Results. Animals in both groups were capable of plantar weight-bearing stepping at speed up to 0.8 m/s as early as 3 weeks and locomotor capabilities were similar at 3 and 5 weeks for both types of graft. Conclusions. Even without locomotor training, either BDNF or NT-3 only producing grafts promote locomotor recovery in complete spinal animals. More clinically applicable delivery methods need to be developed. CI - (c) The Author(s) 2014. FAU - Ollivier-Lanvin, Karen AU - Ollivier-Lanvin K AD - PPD France SAS, Ivry sur Seine, France. FAU - Fischer, Itzhak AU - Fischer I AD - Drexel University College of Medicine, Philadelphia, PA, USA. FAU - Tom, Veronica AU - Tom V AD - Drexel University College of Medicine, Philadelphia, PA, USA. FAU - Houle, John D AU - Houle JD AD - Drexel University College of Medicine, Philadelphia, PA, USA. FAU - Lemay, Michel A AU - Lemay MA AD - Temple University, Philadelphia, PA, USA mlemay@temple.edu. LA - eng GR - NS055976/NS/NINDS NIH HHS/United States GR - NS007440/NS/NINDS NIH HHS/United States GR - R01 NS048844/NS/NINDS NIH HHS/United States GR - NS048844/NS/NINDS NIH HHS/United States GR - T32 NS007440/NS/NINDS NIH HHS/United States GR - P01 NS055976/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20140506 PL - United States TA - Neurorehabil Neural Repair JT - Neurorehabilitation and neural repair JID - 100892086 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neurotrophin 3) SB - IM MH - Animals MH - Biomechanical Phenomena MH - Brain-Derived Neurotrophic Factor/metabolism/*therapeutic use MH - Cats MH - Disease Models, Animal MH - Exercise Test MH - Female MH - Fibroblasts/*metabolism/*transplantation MH - Locomotion MH - Neurotrophin 3/metabolism/*therapeutic use MH - Recovery of Function/*physiology MH - Spinal Cord Injuries/physiopathology/*surgery MH - Time Factors MH - Transduction, Genetic PMC - PMC4223019 MID - NIHMS582266 OTO - NOTNLM OT - kinematics OT - locomotion OT - neurotrophin OT - plasticity OT - spinal cord injury EDAT- 2014/05/08 06:00 MHDA- 2015/08/20 06:00 PMCR- 2016/01/01 CRDT- 2014/05/08 06:00 PHST- 2014/05/08 06:00 [entrez] PHST- 2014/05/08 06:00 [pubmed] PHST- 2015/08/20 06:00 [medline] PHST- 2016/01/01 00:00 [pmc-release] AID - 1545968314532834 [pii] AID - 10.1177/1545968314532834 [doi] PST - ppublish SO - Neurorehabil Neural Repair. 2015 Jan;29(1):90-100. doi: 10.1177/1545968314532834. Epub 2014 May 6.