PMID- 24803807
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20211021
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 20
IP  - 17
DP  - 2014 May 7
TI  - Naofen promotes TNF-alpha-mediated apoptosis of hepatocytes by activating caspase-3 
      in lipopolysaccharide-treated rats.
PG  - 4963-71
LID - 10.3748/wjg.v20.i17.4963 [doi]
AB  - AIM: To investigate whether naofen is involved in tumor necrosis factor 
      (TNF)-alpha-mediated apoptosis of hepatocytes induced by lipopolysaccharide (LPS). 
      METHODS: In vivo, rats were treated with LPS or anti-TNF-alpha antibody, whereas in 
      vitro, primary hepatocytes and Kupffer cells (KCs) were separately isolated from 
      rat livers using collagenase perfusion, and primary hepatocytes were cultured in 
      medium containing LPS or TNF-alpha, or in conditioned medium from LPS-treated KCs 
      (KC-CM)/KC-CM + anti-TNF-alpha antibody. Naofen and TNF-alpha mRNA expression was 
      examined by real-time reverse transcription-polymerase chain reaction. 
      Immunoblotting was used to measure protein expression. Hepatocyte apoptosis was 
      determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) assay. RESULTS: LPS significantly induced both naofen expression 
      and caspase-3 activity in the rat liver, which coincided with an increase in the 
      number of TUNEL-positive hepatocytes. The increase of TNF-alpha expression induced by 
      LPS was preceded by increases in naofen and caspase-3 activity. Elevation of 
      naofen expression and caspase-3 activity was abrogated by pretreatment with 
      anti-TNF-alpha antibody. In KCs, LPS caused an increase in TNF-alpha that was almost 
      consistent with that in the liver of LPS-treated rats. In hepatocytes, neither 
      LPS nor TNF-alpha alone affected either naofen expression or caspase-3 activation. 
      The incubation of hepatocytes with KC-CM significantly enhanced both naofen 
      expression and caspase-3 activity. Moreover, the effects of the KC-CM-induced 
      increase in naofen expression and caspase-3 activity were blocked by anti-TNF-alpha 
      antibody. CONCLUSION: TNF-alpha released from KCs treated with LPS may induce hepatic 
      naofen expression, which then stimulates hepatocellular apoptosis through 
      activation of caspase-3.
FAU - Fan, Jun-Hua
AU  - Fan JH
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
FAU - Feng, Guo-Gang
AU  - Feng GG
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
FAU - Huang, Lei
AU  - Huang L
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
FAU - Tang, Guo-Duo
AU  - Tang GD
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
FAU - Jiang, Hai-Xing
AU  - Jiang HX
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
FAU - Xu, Jing
AU  - Xu J
AD  - Jun-Hua Fan, Guo-Duo Tang, Hai-Xing Jiang, Department of Gastroenterology, the 
      First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi 
      Zhuang Autonomous Region, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (WDR35 protein, rat)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Caspase 3/*metabolism
MH  - Cells, Cultured
MH  - Enzyme Activation
MH  - Hepatocytes/*drug effects/immunology/metabolism/pathology
MH  - Kupffer Cells/*drug effects/immunology/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Paracrine Communication/drug effects
MH  - Proteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
PMC - PMC4009528
OTO - NOTNLM
OT  - Apoptosis
OT  - Caspase-3
OT  - Kupffer cells
OT  - Lipopolysaccharide
OT  - Naofen
OT  - Tumor necrosis factor-alpha
EDAT- 2014/05/08 06:00
MHDA- 2015/04/10 06:00
PMCR- 2014/05/07
CRDT- 2014/05/08 06:00
PHST- 2013/10/25 00:00 [received]
PHST- 2013/12/25 00:00 [revised]
PHST- 2014/03/05 00:00 [accepted]
PHST- 2014/05/08 06:00 [entrez]
PHST- 2014/05/08 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
PHST- 2014/05/07 00:00 [pmc-release]
AID - 10.3748/wjg.v20.i17.4963 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2014 May 7;20(17):4963-71. doi: 10.3748/wjg.v20.i17.4963.