PMID- 24803988
OWN - NLM
STAT- MEDLINE
DCOM- 20141228
LR  - 20211021
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2014
DP  - 2014
TI  - Olive (Olea europaea) leaf extract induces apoptosis and monocyte/macrophage 
      differentiation in human chronic myelogenous leukemia K562 cells: insight into 
      the underlying mechanism.
PG  - 927619
LID - 10.1155/2014/927619 [doi]
LID - 927619
AB  - Differentiation therapy is an attractive approach aiming at reversing malignancy 
      and reactivating endogenous differentiation programs in cancer cells. Olive leaf 
      extract, known for its antioxidant activity, has been demonstrated to induce 
      apoptosis in several cancer cells. However, its differentiation inducing 
      properties and the mechanisms involved are still poorly understood. In this 
      study, we investigated the effect of Chemlali Olive Leaf Extract (COLE) for its 
      potential differentiation inducing effect on multipotent leukemia K562 cells. 
      Results showed that COLE inhibits K562 cells proliferation and arrests the cell 
      cycle at G0/G1, and then at G2/M phase over treatment time. Further analysis 
      revealed that COLE induces apoptosis and differentiation of K562 cells toward the 
      monocyte lineage. Microarray analysis was conducted to investigate the underlying 
      mechanism of COLE differentiation inducing effect. The differentially expressed 
      genes such as IFI16, EGR1, NFYA, FOXP1, CXCL2, CXCL3, and CXCL8 confirmed the 
      commitment of K562 cells to the monocyte/macrophage lineage. Thus our results 
      provide evidence that, in addition to apoptosis, induction of differentiation is 
      one of the possible therapeutic effects of olive leaf in cancer cells.
FAU - Samet, Imen
AU  - Samet I
AUID- ORCID: 0000-0002-9865-4023
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 
      Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
FAU - Han, Junkyu
AU  - Han J
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 
      1-1-1, Tsukuba, Ibaraki 305-8572, Japan ; Alliance of Research on North Africa, 
      University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
FAU - Jlaiel, Lobna
AU  - Jlaiel L
AD  - Laboratory of Environmental Bioprocesses, Biotechnology Center of Sfax, Sfax 
      3018, Tunisia.
FAU - Sayadi, Sami
AU  - Sayadi S
AD  - Laboratory of Environmental Bioprocesses, Biotechnology Center of Sfax, Sfax 
      3018, Tunisia.
FAU - Isoda, Hiroko
AU  - Isoda H
AD  - Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 
      1-1-1, Tsukuba, Ibaraki 305-8572, Japan ; Alliance of Research on North Africa, 
      University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140406
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Cycle Checkpoints/drug effects
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - K562 Cells
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/metabolism/pathology
MH  - Monocytes/cytology/drug effects
MH  - Olea/*chemistry/metabolism
MH  - Plant Extracts/chemistry/*pharmacology
MH  - Plant Leaves/chemistry/metabolism
MH  - Transcriptome/drug effects
PMC - PMC3997986
EDAT- 2014/05/08 06:00
MHDA- 2014/12/30 06:00
PMCR- 2014/04/06
CRDT- 2014/05/08 06:00
PHST- 2013/12/06 00:00 [received]
PHST- 2014/02/16 00:00 [accepted]
PHST- 2014/05/08 06:00 [entrez]
PHST- 2014/05/08 06:00 [pubmed]
PHST- 2014/12/30 06:00 [medline]
PHST- 2014/04/06 00:00 [pmc-release]
AID - 10.1155/2014/927619 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2014;2014:927619. doi: 10.1155/2014/927619. Epub 2014 Apr 
      6.