PMID- 24804376
OWN - NLM
STAT- MEDLINE
DCOM- 20150520
LR  - 20181202
IS  - 1004-5619 (Print)
IS  - 1004-5619 (Linking)
VI  - 30
IP  - 1
DP  - 2014 Feb
TI  - [Relationship between PMI and relative expression of myocardial various RNAs in 
      rats died of different causes].
PG  - 7-12
AB  - OBJECTIVE: To observe the changes of relative expression of myocardial various 
      RNAs in rats died of different causes and their relationship with PMI. METHODS: 
      The rat models were established in which the rats were sacrificed by broken neck, 
      asphyxia, and hemorrhagic shock. Total RNAs were extracted from myocardium. The 
      quantitative real time PCR was used to calculate threshold cycle values of RNAs 
      including glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-actin, inducible 
      nitric oxide synthase (iNOS), hypoxia-inducible factor-1 (HIF-1), tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-6 (IL-6), and U6 small nuclear RNA (U6 
      snRNA) and to study the changes of the relative expressions of various indexes 
      with PMI. RESULTS: U6 snRNA with stable expression level could be used as 
      appropriate internal control. In the early PMI, the relative expression of GAPDH, 
      HIF-1, iNOS, TNF-alpha, and IL-6 more characteristically increased in groups of 
      asphyxia and hemorrhagic shock than in group of broken neck, but the quantity of 
      beta-actin decreased in all groups. In the late PMI, all the relative expressions 
      significantly declined in correlation with the degradation of RNA. CONCLUSION: 
      The characteristic changes of each RNA expression can be used as references to 
      estimate PMI in deaths by different causes.
FAU - Lu, Ye-Hui
AU  - Lu YH
FAU - Zhang, Heng
AU  - Zhang H
FAU - Pan, Hui
AU  - Pan H
FAU - Ma, Kai-Jun
AU  - Ma KJ
FAU - Li, Wen-Can
AU  - Li WC
FAU - Chen, Wen-Feng
AU  - Chen WF
FAU - Jiang, Jie-Qing'
AU  - Jiang JQ
FAU - Xue, Ai-Min
AU  - Xue AM
FAU - Zhang, Ping
AU  - Zhang P
FAU - Wang, Hui-Jun
AU  - Wang HJ
FAU - Chen, Long
AU  - Chen L
LA  - chi
PT  - Journal Article
PL  - China
TA  - Fa Yi Xue Za Zhi
JT  - Fa yi xue za zhi
JID - 9426151
RN  - 0 (Actins)
RN  - 0 (Cytokines)
RN  - 0 (Enzymes)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (U6 small nuclear RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Actins
MH  - Animals
MH  - Cause of Death
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Enzymes/*metabolism
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases
MH  - Myocardium/*metabolism
MH  - Nitric Oxide Synthase Type II
MH  - RNA/*metabolism
MH  - RNA, Small Nuclear
MH  - Rats
MH  - Shock, Hemorrhagic
MH  - Tumor Necrosis Factor-alpha
EDAT- 2014/05/09 06:00
MHDA- 2015/05/21 06:00
CRDT- 2014/05/09 06:00
PHST- 2014/05/09 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/05/21 06:00 [medline]
PST - ppublish
SO  - Fa Yi Xue Za Zhi. 2014 Feb;30(1):7-12.