PMID- 24804377
OWN - NLM
STAT- MEDLINE
DCOM- 20150520
LR  - 20211203
IS  - 1004-5619 (Print)
IS  - 1004-5619 (Linking)
VI  - 30
IP  - 1
DP  - 2014 Feb
TI  - [Endoplasmic reticulum stress mediates lipopolysaccharide-induced apoptosis in 
      rat hepatocyte].
PG  - 13-8
AB  - OBJECTIVE: To investigate the role of endoplasmic reticulum stress (ERS) in 
      lipopolysaccharide (LPS)-induced hepatocyte apoptosis. METHODS: Cells of the rat 
      hepatocyte line BRL were cultured. The hepatocytes were treated with LPS, ERS 
      inducer thapsigargin (TG), and ERS inhibitor 4-phenylbutyric acid (4-PBA), 
      respectively or in their different combination. The cell viability was measured 
      by MTT assay. The cyto-nuclear morphological changes of apoptosis cells were 
      detected by the fluorescent dye Hoechst 33258. The apoptosis rate was assessed by 
      flow cytometry with Annexin V-FITC/PI double-staining. Expressions of GRP78 as 
      ERS marker protein, CHOP, caspase-12 and cleaved-caspase-3 as ERS related protein 
      were detected by Western blotting. RESULTS: LPS could cause a decrease in cell 
      viability and an increase in apoptosis rate in a dose- and time-dependent manner. 
      The expression of GRP78, CHOP, caspase-12 and cleaved-caspase-3 proteins were 
      significantly increased with LPS treatment. TG led to a marked decrease in cell 
      viability and an increase in apoptosis rate, which aggravated the hepatocyte 
      injury induced by LPS; whereas 4-PBA alleviated LPS-induced apoptosis. 
      CONCLUSION: ERS mediates LPS-induced hepatocyte injuries, indicating that ERS may 
      play a vital role in the pathogenesis of LPS-induced hepatocyte injuries.
FAU - Ji, Ying-Lei
AU  - Ji YL
FAU - Yan, Jun
AU  - Yan J
FAU - Wang, Yan-Sha
AU  - Wang YS
FAU - Liu, Yi-Chang
AU  - Liu YC
FAU - Gu, Zhen-Yong
AU  - Gu ZY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Fa Yi Xue Za Zhi
JT  - Fa yi xue za zhi
JID - 9426151
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Phenylbutyrates)
RN  - 7WY7YBI87E (4-phenylbutyric acid)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Caspase 3
MH  - Cell Survival
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - *Endoplasmic Reticulum Stress
MH  - Heat-Shock Proteins
MH  - Hepatocytes
MH  - Lipopolysaccharides
MH  - Phenylbutyrates
MH  - Rats
EDAT- 2014/05/09 06:00
MHDA- 2015/05/21 06:00
CRDT- 2014/05/09 06:00
PHST- 2014/05/09 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/05/21 06:00 [medline]
PST - ppublish
SO  - Fa Yi Xue Za Zhi. 2014 Feb;30(1):13-8.