PMID- 24805903
OWN - NLM
STAT- MEDLINE
DCOM- 20150126
LR  - 20220331
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 15
IP  - 6
DP  - 2014 Jun 9
TI  - Differentiation of tonsil-tissue-derived mesenchymal stem cells controlled by 
      surface-functionalized microspheres in PEG-polypeptide thermogels.
PG  - 2180-7
LID - 10.1021/bm500342r [doi]
AB  - Poly(ethylene glycol)-poly(l-alanine) diblock copolymer (PEG-L-PA; molecular 
      weight of each block of 1000-1080 Da) aqueous solutions undergo sol-to-gel 
      transition in a 3.0-8.0 wt % concentration range as the temperature increases. By 
      incorporating the polystyrene microspheres with different functional groups with 
      a size of 100-800 mum in in situ formed PEG-L-PA thermogels, the differentiation 
      of tonsil-tissue-derived mesenchymal stem cells (TMSCs) was investigated. The 
      mRNA expression and immunohistochemical assays suggested that the TMSCs 
      preferentially undergo adipogenesis in the ammonium (-NH3(+))- or thiol 
      (-SH)-functionalized microsphere incorporated thermogels; chondrogenesis in the 
      thiol-, phosphate (PO3(2-))-, or carboxylate (-COO(-))-functionalized microsphere 
      incorporated thermogels; and osteogenesis in the phosphate-, 
      carboxylate-functionalized, or neat polystyrene microsphere incorporated 
      thermogels. This paper provides a new TMSC 3D culture system of a sol-gel 
      reversible matrix and suggests that the surface-functional groups of microspheres 
      in the thermogel can control the preferential differentiation of stem cells into 
      specific cell types during the 3D culture.
FAU - Kye, Eun Jeong
AU  - Kye EJ
AD  - Department of Chemistry and Nano Science, Ewha Global Top 5 Research Program, 
      Ewha Womans University , 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 120-750, Korea.
FAU - Kim, Seung-Jin
AU  - Kim SJ
FAU - Park, Min Hee
AU  - Park MH
FAU - Moon, Hyo Jung
AU  - Moon HJ
FAU - Ryu, Kyung Ha
AU  - Ryu KH
FAU - Jeong, Byeongmoon
AU  - Jeong B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Gels)
RN  - 0 (Peptides)
RN  - 25191-17-7 (polyalanine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cells, Cultured
MH  - Gels
MH  - Humans
MH  - Mesenchymal Stem Cells/*drug effects/physiology
MH  - *Microspheres
MH  - Palatine Tonsil/cytology/*drug effects/physiology
MH  - Peptides/chemistry/*pharmacology
MH  - Polyethylene Glycols/chemistry/*pharmacology
MH  - Surface Properties/drug effects
EDAT- 2014/05/09 06:00
MHDA- 2015/01/27 06:00
CRDT- 2014/05/09 06:00
PHST- 2014/05/09 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
AID - 10.1021/bm500342r [doi]
PST - ppublish
SO  - Biomacromolecules. 2014 Jun 9;15(6):2180-7. doi: 10.1021/bm500342r. Epub 2014 May 
      14.