PMID- 24806622
OWN - NLM
STAT- MEDLINE
DCOM- 20141211
LR  - 20220318
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 290
IP  - 4
DP  - 2014 Oct
TI  - The effect of hesperetin on ischemia-reperfusion injury in rat ovary.
PG  - 763-9
LID - 10.1007/s00404-014-3267-8 [doi]
AB  - PURPOSE: Hesperidin (HES), a citrus fruit extract, has beneficial effects on 
      various ischemia/reperfusion (I/R) models. We aimed to evaluate the possible 
      positive effects of hesperetin (HPT), an active metabolite of HES, on a rat 
      ovarian I/R model. METHODS: We divided 24 Wistar Albino rats into four groups. 
      Group I (n = 6) was sham operated, Group II (n = 6) was the I/R group, Group III 
      (n = 6) was the I/R + solvent group and Group IV (n = 6) was the I/R + HPT group. 
      Three hours of ischemia and 3 h of reperfusion were performed on each rat in 
      Groups II, III, and IV. Dimethyl sulfoxide (DMSO) was given intraperitoneally to 
      the rats in the III. Group, and 50 mg/kg of HPT dissolved in DMSO was given 
      intraperitoneally to the rats in the IV. Group 30 min before reperfusion. After 
      3 h of reperfusion, the ipsilateral ovaries of the rats were examined 
      immunohistochemically to detect apoptosis. RESULTS: Hematoxylin and eosin (H and 
      E) staining demonstrated less edema and hemorrhage in the group where HPT was 
      applied. Caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL) staining showed significantly lower apoptosis in the group 
      where HPT was used when compared to either the I/R or solvent group. CONCLUSIONS: 
      To the best of our knowledge, this is the first study that shows the beneficial 
      effects of HPT in an ovarian I/R injury. HPT improved tissue damage and apoptosis 
      caused by I/R injury. To identify the possible positive effects of HPT in ovarian 
      torsion of humans and use in clinical practice, more studies must be performed.
FAU - Cakir Gungor, Ayse Nur
AU  - Cakir Gungor AN
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Canakkale Onsekiz 
      Mart University, Canakkale, Turkey, aysenurcakirgungor@gmail.com.
FAU - Gencer, Meryem
AU  - Gencer M
FAU - Karaca, Turan
AU  - Karaca T
FAU - Hacivelioglu, Servet
AU  - Hacivelioglu S
FAU - Uysal, Ahmet
AU  - Uysal A
FAU - Korkmaz, Fatma
AU  - Korkmaz F
FAU - Demirtas, Selim
AU  - Demirtas S
FAU - Cosar, Emine
AU  - Cosar E
LA  - eng
PT  - Journal Article
DEP - 20140508
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
RN  - 0 (Solvents)
RN  - E750O06Y6O (Hesperidin)
RN  - EC 3.4.22.- (Caspase 3)
RN  - Q9Q3D557F1 (hesperetin)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Caspase 3/metabolism
MH  - Dimethyl Sulfoxide
MH  - Edema/pathology
MH  - Female
MH  - Hemorrhage/pathology
MH  - Hesperidin/*pharmacology
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Ovary/*blood supply/*pathology
MH  - Rats, Wistar
MH  - *Reperfusion
MH  - Reperfusion Injury/*therapy
MH  - Solvents
MH  - Torsion, Mechanical
EDAT- 2014/05/09 06:00
MHDA- 2014/12/17 06:00
CRDT- 2014/05/09 06:00
PHST- 2014/01/11 00:00 [received]
PHST- 2014/04/17 00:00 [accepted]
PHST- 2014/05/09 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2014/12/17 06:00 [medline]
AID - 10.1007/s00404-014-3267-8 [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2014 Oct;290(4):763-9. doi: 10.1007/s00404-014-3267-8. Epub 
      2014 May 8.