PMID- 2480745 OWN - NLM STAT- MEDLINE DCOM- 19900123 LR - 20220722 IS - 0166-3542 (Print) IS - 0166-3542 (Linking) VI - 12 IP - 2 DP - 1989 Sep TI - Inhibition of reverse transcriptases by flavonoids. PG - 99-110 AB - Selected naturally occurring flavonoids were shown to inhibit three reverse transcriptases (RT): avian myeloblastosis (AMV) RT, Rous-associated virus-2 (RAV-2) RT and Maloney murine leukemia virus (MMLV) RT when poly (rA)oligo(dT)12-18 or rabbit globin mRNA were used as template. Amentoflavone, scutellarein and quercetin were the most active compounds and their effect was concentration-dependent. The enzymes exhibited differential sensitivity to the inhibitory effects of the flavonoids. The compounds also inhibited rabbit globin mRNA-directed, MMLV RT-catalyzed DNA synthesis. Amentoflavone and scutellarein inhibited ongoing new DNA synthesis catalyzed by RAV-2 RT. Kinetic studies were performed in an attempt to elucidate the mechanism of action of amentoflavone and scutellarein. A model is proposed for the mechanism of action of the flavonoids on RT activity. FAU - Spedding, G AU - Spedding G AD - Department of Medicine, State University of New York, Buffalo General Hospital 14203. FAU - Ratty, A AU - Ratty A FAU - Middleton, E Jr AU - Middleton E Jr LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Antiviral Res JT - Antiviral research JID - 8109699 RN - 0 (Biflavonoids) RN - 0 (Flavanones) RN - 0 (Flavonoids) RN - 0 (Reverse Transcriptase Inhibitors) RN - 9I1VC79L77 (amentoflavone) RN - 9IKM0I5T1E (Quercetin) RN - P460GTI853 (scutellarein) SB - IM MH - Animals MH - Avian Leukosis Virus/enzymology MH - Avian Myeloblastosis Virus/enzymology MH - *Biflavonoids MH - *Flavanones MH - Flavonoids/*pharmacology MH - Kinetics MH - Molecular Structure MH - Moloney murine leukemia virus/enzymology MH - Quercetin/*pharmacology MH - Retroviridae/*enzymology MH - *Reverse Transcriptase Inhibitors EDAT- 1989/09/01 00:00 MHDA- 2001/03/28 10:01 CRDT- 1989/09/01 00:00 PHST- 1989/09/01 00:00 [pubmed] PHST- 2001/03/28 10:01 [medline] PHST- 1989/09/01 00:00 [entrez] AID - 0166-3542(89)90073-9 [pii] AID - 10.1016/0166-3542(89)90073-9 [doi] PST - ppublish SO - Antiviral Res. 1989 Sep;12(2):99-110. doi: 10.1016/0166-3542(89)90073-9.