PMID- 24809310
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20221207
IS  - 1435-702X (Electronic)
IS  - 0721-832X (Linking)
VI  - 252
IP  - 12
DP  - 2014 Dec
TI  - Association of monocyte chemoattractant protein-1 (MCP-1)2518A/G polymorphism 
      with proliferative diabetic retinopathy in northern Chinese type 2 diabetes.
PG  - 1921-6
LID - 10.1007/s00417-014-2651-1 [doi]
AB  - BACKGROUND: The pathogenesis of proliferative diabetic retinopathy (PDR) remains 
      poorly understood. Recent studies have implicated that monocyte chemoattractant 
      protein-1 (MCP-1) is associated with diabetic microvascular or macrovascular 
      complications. However, the relationship between single nucleotide 
      polymorphism(SNP)c.2518A/G -rs1024611 in the MCP-1 gene with diabetic retinopathy 
      remains controversial. In the present study, we evaluated the association of SNP 
      in the MCP-1 gene with diabetic retinopathy (DR) and diabetic macular edema (DME) 
      in a Chinese population from Northern China with type 2 diabetes. METHODS: We 
      conducted a case-control study, which enrolled 1,043 subjects with type 2 
      diabetes (528 with DR, including 277PDR; 515 without DR), and SNP genotyping of 
      c.2518A/G in the MCP-1 gene was performed using the polymerase chain reaction. 
      Genomic DNA was isolated from 3 ml samples of whole blood using a modified 
      conventional DNA extraction method. The genotype and allele frequencies of 
      2518A/G were studied by using an automated DNA sequencer (ABI PRISM 3730 DNA 
      Sequencer). RESULTS: The demographic and clinical characteristics did not differ 
      among genotype subgroups. The MCP-1(-2518) GG genotype was significantly 
      associated with DR susceptibility with OR of 1.481 (95 % CI, 1.019-2.153) 
      (P = 0.046). There were no significant differences in the MCP-1(-2518) G allele 
      frequencies in DR compared to non-diabetic retinopathy (DNR) (P > 0.05, 
      OR = 0.841, 95 % CI, 0.705-1.002). The MCP-1(-2518) GG genotype was significantly 
      associated with high-risk PDR susceptibility with OR of 2.656 (95 % CI, 
      1.222-5.775) (P = 0.014). The MCP-1(-2518) G allele was significantly increased 
      in high-risk PDR patients (P = 0.020, OR = 1.481, 95 % CI, 1.070-2.051) compared 
      with A allele. Genotype and allele frequencies of various DME of the DR patients 
      were compared, but there were no significant associations established (P > 0.05). 
      CONCLUSIONS: It is likely that the MCP-1 c.2518G/G genotype is a susceptibility 
      gene for DR in Chinese type 2 diabetic patients, especially the high-risk PDR. 
      There is no association with DME and c.2518G/G.
FAU - Dong, Li
AU  - Dong L
AD  - Key Laboratory of Harbin Medical University Eye Center, Eye Hospital, First 
      Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang 
      District, Harbin, 150001, Heilongjiang Province, People's Republic of China.
FAU - Lv, Xiao Ying
AU  - Lv XY
FAU - Wang, Bin Jie
AU  - Wang BJ
FAU - Wang, Ye Qing
AU  - Wang YQ
FAU - Mu, Hua
AU  - Mu H
FAU - Feng, Zhuo Lei
AU  - Feng ZL
FAU - Liu, Ping
AU  - Liu P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140509
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Asian People/*genetics
MH  - Case-Control Studies
MH  - Chemokine CCL2/*genetics
MH  - China/epidemiology
MH  - Diabetes Mellitus, Type 2/*genetics
MH  - Diabetic Retinopathy/diagnosis/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Genotyping Techniques
MH  - Humans
MH  - Macular Edema/diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Single Nucleotide
EDAT- 2014/05/09 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/05/10 06:00
PHST- 2013/06/20 00:00 [received]
PHST- 2014/04/14 00:00 [accepted]
PHST- 2014/04/09 00:00 [revised]
PHST- 2014/05/10 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - 10.1007/s00417-014-2651-1 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2014 Dec;252(12):1921-6. doi: 
      10.1007/s00417-014-2651-1. Epub 2014 May 9.