PMID- 2480987
OWN - NLM
STAT- MEDLINE
DCOM- 19900130
LR  - 20210222
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 30
IP  - 9
DP  - 1989 Sep
TI  - High carbohydrate fat-free diet modulates epitope expression of LDL-apoB-100 and 
      interaction of LDL with human fibroblasts.
PG  - 1131-9
AB  - High carbohydrate diets are known to increase the concentration of very low 
      density lipoprotein (VLDL) and to lower the concentrations of low density 
      lipoprotein (LDL) and high density lipoprotein (HDL) in plasma. Such diets also 
      alter lipoprotein compositions and metabolism. The aims of the present study were 
      to assess in detail the effects of a virtually fat-free high carbohydrate (CHO) 
      diet (CHO greater than 85% and fat less than 1% of calories) on various aspects 
      of LDL. Thirteen healthy normolipidemic volunteers ate a basal "American" diet 
      and the CHO diet for 7 days each in a forward or reverse sequence. Fasting blood 
      samples were drawn at the ends of each study period and analyzed for lipoprotein 
      lipid and apolipoprotein concentrations. Compositions of LDL particles isolated 
      by ultracentrifugation were characterized chemically, LDL sizes were assessed by 
      nondenaturing gradient electrophoresis on 2-16% gels, and association and 
      degradation of LDL with normal human skin fibroblasts were quantified in cell 
      cultures. Immunoreactivities of apoB in LDL were tested in solid phase 
      competitive binding radioimmunoassays using five monoclonal anti-LDL antibodies 
      that reacted with defined epitopes of apoB-100. The study diet produced 
      consistent decreases of LDL cholesterol and apoB concentrations by 25% and 17%, 
      respectively. LDL compositions were altered. Mean LDL triglycerides increased 3% 
      to 4% of total LDL mass (P less than 0.004), and LDL particle sizes decreased (P 
      less than 0.01). In radioimmunoassays that contained monoclonal antibody B1B3, an 
      antibody that inhibits binding of LDL to the LDL receptor, the mean ED50 value 
      for LDL protein was reduced from 3.75 to 2.66 micrograms (P less than 
      0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Keidar, S
AU  - Keidar S
AD  - Department of Medicine, Washington University School of Medicine, St. Louis, MO 
      63110.
FAU - Goldberg, A C
AU  - Goldberg AC
FAU - Cook, K
AU  - Cook K
FAU - Bateman, J
AU  - Bateman J
FAU - Schonfeld, G
AU  - Schonfeld G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Apolipoprotein B-100)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Epitopes)
RN  - 0 (Lipoproteins)
RN  - 0 (Lipoproteins, LDL)
SB  - IM
MH  - Adult
MH  - Apolipoprotein B-100
MH  - Apolipoproteins B/*blood/immunology
MH  - Cells, Cultured
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Dietary Fats/*administration & dosage
MH  - Epitopes
MH  - Female
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Lipoproteins/blood
MH  - Lipoproteins, LDL/*blood/immunology/metabolism
MH  - Male
EDAT- 1989/09/01 00:00
MHDA- 1989/09/01 00:01
CRDT- 1989/09/01 00:00
PHST- 1989/09/01 00:00 [pubmed]
PHST- 1989/09/01 00:01 [medline]
PHST- 1989/09/01 00:00 [entrez]
AID - S0022-2275(20)38250-X [pii]
PST - ppublish
SO  - J Lipid Res. 1989 Sep;30(9):1131-9.