PMID- 24811215
OWN - NLM
STAT- MEDLINE
DCOM- 20150112
LR  - 20140606
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 27
IP  - 5
DP  - 2014 Jun
TI  - Gene expression profiling of immune responsive and fibrosis genes in hepatitis C 
      virus infected patients.
PG  - 250-4
LID - 10.1089/vim.2014.0005 [doi]
AB  - Hepatitis C virus (HCV) is a dreadful viral disease, responsible for 170 million 
      cases worldwide, of which most are from Asia and Africa and approximately 10 
      million people are from Pakistan. Currently, the pegylated interferon alpha 
      (PEG-INF-alpha) has been approved as the standard of care in combination with 
      ribavirin and Boceprevir/Telaprevir. Many studies regarding gene expression 
      analysis of liver biopsy samples of patients with chronic HCV infection have been 
      carried out previously. However, there are very few reports of expression 
      analysis carried out using blood samples of HCV patients. Therefore, in this 
      study, gene expression of human immune responsive genes (MMP-9, OAS1) and 
      fibrogenic responsive gene (KRT19) was done in the peripheral blood mononuclear 
      cells (PBMCs) of chronic HCV infected patients having differences in viral 
      titers. Blood samples were collected from different hospitals in Pakistan. RNA 
      was extracted and cDNA was synthesized according to the protocol prescribed by 
      the Enzynomics M-MLV Reverse Transcriptase((R)) Kit. The synthesized cDNA was 
      amplified through polymerase chain reaction (PCR) using specific primers of 
      immune responsive genes. The results were further evaluated using real-time PCR. 
      There was a significant increase in the expression of the immune responsive genes 
      (MMP-9, OAS1, CXCL6, CXCR3, ApoA1, and MYC) of HCV genotype 3a patients compared 
      to controls. Similarly, the expression of the fibrosis genes was upregulated in 
      HCV genotype 3a patients compared to controls. The information gained through 
      this study is helpful to identify a noninvasive marker to determine liver 
      fibrosis, and may also give useful information to understand HCV pathogenesis and 
      develop better therapeutic regimens.
FAU - Idrees, Sobia
AU  - Idrees S
AD  - 1 Department of Bioinformatics and Biotechnology, Government College University , 
      Faisalabad, Pakistan .
FAU - Ashfaq, Usman Ali
AU  - Ashfaq UA
FAU - Masoud, Muhammad Shareef
AU  - Masoud MS
FAU - Qasim, Muhammad
AU  - Qasim M
FAU - Javed, Tariq
AU  - Javed T
FAU - Ali, Asad
AU  - Ali A
LA  - eng
PT  - Journal Article
DEP - 20140508
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Gene Expression Profiling
MH  - Hepacivirus/isolation & purification
MH  - Hepatitis C, Chronic/*complications/*genetics
MH  - Hospitals
MH  - Humans
MH  - Leukocytes, Mononuclear
MH  - Liver Cirrhosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Pakistan
MH  - Real-Time Polymerase Chain Reaction
MH  - Viral Load
MH  - Young Adult
EDAT- 2014/05/09 06:00
MHDA- 2015/01/13 06:00
CRDT- 2014/05/10 06:00
PHST- 2014/05/10 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
AID - 10.1089/vim.2014.0005 [doi]
PST - ppublish
SO  - Viral Immunol. 2014 Jun;27(5):250-4. doi: 10.1089/vim.2014.0005. Epub 2014 May 8.