PMID- 24811302
OWN - NLM
STAT- MEDLINE
DCOM- 20150313
LR  - 20220318
IS  - 1724-6040 (Electronic)
IS  - 0391-3988 (Linking)
VI  - 37
IP  - 5
DP  - 2014 May
TI  - Alpinia oxyphylla Miquel fruit extract activates MAPK-mediated signaling of PAs 
      and MMP2/9 to induce Schwann cell migration and nerve regeneration.
PG  - 402-13
LID - 10.5301/ijao.5000313 [doi]
AB  - OBJECTIVES: This study investigates the molecular mechanisms by which Alpiniae 
      oxyphyllae fructus (AOF) promotes neuron regeneration. METHODS: A piece of 
      silicone rubber was guided across a 15 mm gap in the sciatic nerve of a rat. This 
      nerve gap was then filled with different concentrations of AOF extract (0-200 
      mg/ml). We investigated the role of MAPK (ERK1/2, JNK and p38) pathways for 
      AOF-induced matrix-degrading proteolytic enzyme (PAs and MMP2/9) production in 
      RSC96 Schwann cells. RESULTS: The results showed that AOF increased the 
      expressions of uPA, tPA, MMP-9, and MAPKs in vivo. In vitro, our results show 
      that treatment with AOF extract induces ERK1/2, JNK, and p38 phosphorylation to 
      activate the downstream PAs and MMPs signaling expression. AOF-stimulated ERK1/2, 
      JNK, and p38 phosphorylation attenuated by individual pretreatment with siRNAs or 
      inhibitors (U0126, SP600125 and SB203580), resulting in migration and uPA-related 
      signal pathway inhibition. CONCLUSIONS: Taken together our data suggests the 
      MAPKs (ERK1/2, JNK and p38), PAs (uPA, tPA), MMP (MMP2, MMP9) regenerative and 
      migration signaling pathway of Schwann cells regulated by AOF extract might play 
      a major role in Schwann cell migration and damaged peripheral nerve regeneration.
FAU - Chang, Yung-Ming
AU  - Chang YM
AD  - 2 Chinese Medicine Department, E-DA Hospital, Kaohsiung - Taiwan.
FAU - Ye, Chi-Xin
AU  - Ye CX
FAU - Ho, Tsung-Jung
AU  - Ho TJ
FAU - Tsai, Te-Neng
AU  - Tsai TN
FAU - Chiu, Ping-Ling
AU  - Chiu PL
FAU - Tsai, Chin-Chuan
AU  - Tsai CC
FAU - Lin, Yueh-Min
AU  - Lin YM
FAU - Kuo, Chia-Hua
AU  - Kuo CH
FAU - Tsai, Fuu-Jen
AU  - Tsai FJ
FAU - Tsai, Chang-Hai
AU  - Tsai CH
FAU - Huang, Chih-Yang
AU  - Huang CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140417
PL  - United States
TA  - Int J Artif Organs
JT  - The International journal of artificial organs
JID - 7802649
RN  - 0 (Alpinia oxyphylla fruit extract)
RN  - 0 (Plant Extracts)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - *Alpinia
MH  - Animals
MH  - Cell Movement/*drug effects/physiology
MH  - MAP Kinase Signaling System/*drug effects/physiology
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Nerve Regeneration/*drug effects/physiology
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Schwann Cells/*drug effects/metabolism
MH  - Tissue Plasminogen Activator/metabolism
MH  - Urokinase-Type Plasminogen Activator/metabolism
EDAT- 2014/05/09 06:00
MHDA- 2015/03/17 06:00
CRDT- 2014/05/10 06:00
PHST- 2014/02/01 00:00 [accepted]
PHST- 2014/05/10 06:00 [entrez]
PHST- 2014/05/09 06:00 [pubmed]
PHST- 2015/03/17 06:00 [medline]
AID - B89294D2-96D8-413E-992F-20547AA8944B [pii]
AID - 10.5301/ijao.5000313 [doi]
PST - ppublish
SO  - Int J Artif Organs. 2014 May;37(5):402-13. doi: 10.5301/ijao.5000313. Epub 2014 
      Apr 17.