PMID- 24813021 OWN - NLM STAT- MEDLINE DCOM- 20150416 LR - 20140602 IS - 1873-2232 (Electronic) IS - 0378-4320 (Linking) VI - 147 IP - 3-4 DP - 2014 Jun 30 TI - The expression of epidermal growth factor receptors and their ligands (epidermal growth factor, neuregulin, amphiregulin) in the bitch uterus during the estrus cycle. PG - 161-79 LID - S0378-4320(14)00114-6 [pii] LID - 10.1016/j.anireprosci.2014.04.005 [doi] AB - In order to study the possible role of EGFR receptors in the bitch reproductive process, we have analyzed the expression pattern and localization of EGFR receptors and some of their ligands epidermal growth factor (EGF), neuregulin (NRG), amphiregulin (AREG), in the uterus during the estrus cycle using immunohistochemistry. The immunostaining for receptors and ligands of EGFR/ligand system was confined to membrane and cytoplasm of the target cells. Variations were observed, not only at the different stages of the estrous cycle, but also in the different tissue compartments of the uterus. However, it was detected that the immunostainings for NRG and AREG in the different cells do not show important differences at stages of the estrus cycle. In the luminal epithelium, strong immunostaining for ErbB1/HER1, ErbB2/HER2, ErbB4/HER4 and EGF was found at estrus. In the glandular epithelium, strong immunostaining for ErbB4/HER4 was observed at diestrus, while strong immunostaining for EGF was detected in both of estrus and diestrus. ErbB3/HER3 immunoreactivity in the stromal cells was higher at diestrus and anestrus, while ErbB4/HER4 immunoreactivity was lower at anestrus. In the myometrium, the highest levels of immunoreactivity of ErbB2/HER2 were found at estrus, while ErbB3/HER3 immunoreactivity was higher at anestrus. EGF immunoreactivity was lower at anestrus compared to other stage of cycle. Altered EGFR/ligand system expression during the estrus cycle suggests this growth factor system is a potent regulator of proliferation and differentiation events during preparation for implantation of bitch uterus. CI - Copyright (c) 2014 Elsevier B.V. All rights reserved. FAU - Sagsoz, Hakan AU - Sagsoz H AD - Dicle University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 21280 Diyarbakir, Turkey. Electronic address: hakan.sagsoz@dicle.edu.tr. FAU - Liman, Narin AU - Liman N AD - Erciyes University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 38039 Kayseri, Turkey. FAU - Saruhan, Berna Guney AU - Saruhan BG AD - Dicle University, Faculty of Veterinary Medicine, Department of Histology and Embryology, 21280 Diyarbakir, Turkey. FAU - Kucukaslan, Ibrahim AU - Kucukaslan I AD - Dicle University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, 21280 Diyarbakir, Turkey. LA - eng PT - Journal Article DEP - 20140423 PL - Netherlands TA - Anim Reprod Sci JT - Animal reproduction science JID - 7807205 RN - 0 (Amphiregulin) RN - 0 (EGF Family of Proteins) RN - 0 (Neuregulin-1) RN - 62229-50-9 (Epidermal Growth Factor) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Amphiregulin/genetics/metabolism MH - Animals MH - *Dogs/genetics/metabolism MH - EGF Family of Proteins/*genetics/metabolism MH - Epidermal Growth Factor/genetics/metabolism MH - ErbB Receptors/*genetics/metabolism MH - Estrous Cycle/genetics/metabolism MH - Estrus/*genetics/metabolism MH - Female MH - Gene Expression MH - Neuregulin-1/genetics/metabolism MH - Uterus/*metabolism OTO - NOTNLM OT - AREG OT - Diestrus OT - Endometrium OT - ErbB OT - Estrus OT - NRG EDAT- 2014/05/13 06:00 MHDA- 2015/04/17 06:00 CRDT- 2014/05/13 06:00 PHST- 2013/12/09 00:00 [received] PHST- 2014/03/14 00:00 [revised] PHST- 2014/04/10 00:00 [accepted] PHST- 2014/05/13 06:00 [entrez] PHST- 2014/05/13 06:00 [pubmed] PHST- 2015/04/17 06:00 [medline] AID - S0378-4320(14)00114-6 [pii] AID - 10.1016/j.anireprosci.2014.04.005 [doi] PST - ppublish SO - Anim Reprod Sci. 2014 Jun 30;147(3-4):161-79. doi: 10.1016/j.anireprosci.2014.04.005. Epub 2014 Apr 23.