PMID- 24819541
OWN - NLM
STAT- MEDLINE
DCOM- 20150331
LR  - 20161125
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 53
DP  - 2014 Aug
TI  - PTEN in smooth muscle cells is essential for colonic immune homeostasis.
PG  - 108-14
LID - S1357-2725(14)00145-9 [pii]
LID - 10.1016/j.biocel.2014.04.029 [doi]
AB  - Colonic immune homeostasis is essential for normal gastrointestinal tract 
      functioning. In this study, we report that specific gene targeting of phosphatase 
      and tensin homolog (PTEN) in smooth muscle cells caused age-related colonic 
      lymphoid hyperplasia followed by global immune activation in mice. Beginning at 5 
      weeks of age, these mutant mice displayed massive neutrophil infiltration in the 
      colonic lamina propria. The gene expression levels of pro-inflammatory cytokines 
      and chemokines, including those code for monocyte chemotactic protein-1 (Mcp-1), 
      stromal cell-derived factor 1alpha (Sdf-1alpha), and chemokine (C-C motif) ligand 28 
      (Ccl-28), were upregulated in the colon. Accordingly, permeability and 
      proliferation of the colonic epithelium was compromised. These abnormalities were 
      alleviated to a great extent when the mutants were crossed with Akt1-null mice, 
      indicating that the pathogenesis was mediated by Akt1 signaling. Our results 
      suggest that in smooth muscle cells, PTEN is crucial for maintaining colonic 
      immune homeostasis.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Qi, Xin
AU  - Qi X
AD  - Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 
      Beijing 100071, PR China; MOE Key Laboratory of Model Animal for Disease Study, 
      Model Animal Research Center, Nanjing University, 12 Xuefu Road, Nanjing 210061, 
      China.
FAU - Xu, Jingyue
AU  - Xu J
AD  - Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 
      Beijing 100071, PR China.
FAU - Gu, Pengyu
AU  - Gu P
AD  - Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 
      Beijing 100071, PR China.
FAU - Yang, Xiao
AU  - Yang X
AD  - Genetic Laboratory of Development and Diseases, Institute of Biotechnology, 
      Beijing 100071, PR China.
FAU - Gao, Xiang
AU  - Gao X
AD  - MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research 
      Center, Nanjing University, 12 Xuefu Road, Nanjing 210061, China. Electronic 
      address: gaoxiang@nju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140510
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL12)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (Pten protein, mouse)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Chemokine CXCL12/biosynthesis
MH  - Colonic Neoplasms/*genetics/immunology/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - Inflammation/*genetics/immunology/pathology
MH  - Mice
MH  - Myocytes, Smooth Muscle/immunology/metabolism/pathology
MH  - Neutrophils/immunology/pathology
MH  - PTEN Phosphohydrolase/*genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Pseudolymphoma/*genetics/pathology
MH  - Signal Transduction/genetics
OTO - NOTNLM
OT  - Akt1
OT  - Immune activation
OT  - Lymphoid follicle
OT  - PTEN
OT  - Smooth muscle cell
EDAT- 2014/05/14 06:00
MHDA- 2015/04/01 06:00
CRDT- 2014/05/14 06:00
PHST- 2013/11/25 00:00 [received]
PHST- 2014/04/14 00:00 [revised]
PHST- 2014/04/29 00:00 [accepted]
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2015/04/01 06:00 [medline]
AID - S1357-2725(14)00145-9 [pii]
AID - 10.1016/j.biocel.2014.04.029 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2014 Aug;53:108-14. doi: 10.1016/j.biocel.2014.04.029. 
      Epub 2014 May 10.