PMID- 24820304
OWN - NLM
STAT- MEDLINE
DCOM- 20141222
LR  - 20140513
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 49
IP  - 3
DP  - 2014 Mar
TI  - [Clinical investigation to compare aCGH and FISH in preimplantationgenetic 
      diagnosis of chromosome translocation carriers].
PG  - 193-8
AB  - OBJECTIVE: To investigate the clinical use of array comparative genomic 
      hybridization (aCGH) with fluorescence in situ hybridization (FISH) in 
      preimplantion genetic diagnosis (PGD) for reciprocal and Robertsonian 
      translocation carriers. METHODS: From Jan. 2012 to Jun. 2013, a total of 220 PGD 
      cycles from 151 reciprocal translocation and 62 Robertsonian translocation 
      carrier couples, including 33 cycles for reciprocal translocation carriers and 22 
      cycles for Robertsonian translocation carriers performed using array CGH, and 119 
      cycles for reciprocal translocation carriers and 46 cycles for Robertsonian 
      translocation carriers performed using FISH were retrospectively studied. The 
      rate of accurate diagnosis was compared between two methods. RESULTS: Normal 
      and/or balance rates of the two translocated chromosomes detected by aCGH for 
      both reciprocal and Robertsonian translocation carriers were 38.20% (123/322) and 
      67.20% (127/189), significantly higher than 15.39% (195/1 267) and 30.75% 
      (202/657) by FISH (all P < 0.05). Abnormal rates of the two translocated 
      chromosomes detected by aCGH for both reciprocal and Robertsonian translocation 
      carriers were 59.32% (191/322) and 30.69% (58/189), significantly lower than 
      83.03% (1 052/1 267) and 67.43% (443/657) by FISH (all P < 0.05). And the rate of 
      aneu ploidy in non-translocated chromosome from reciprocal translocation embryos 
      was 20.19% (65/322), which was significantly lower than 38.62% (13/189) from 
      Robertsonian translocation embryos (P < 0.01). CONCLUSIONS: Normal and/or balance 
      rates of the two translocated chromosomes detected by array CGH were 
      significantly higher than FISH. And the rate of aneuploidy in non-translocated 
      chromosomes from reciprocal translocation embryos was significantly lower than 
      that from Robertsonian translocation embryos.
FAU - Xie, Yanxin
AU  - Xie Y
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Xu, Yanwen
AU  - Xu Y
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Miao, Benyu
AU  - Miao B
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Zeng, Yanhong
AU  - Zeng Y
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Zhou, Canquan
AU  - Zhou C
AD  - Center for Reproductive Medicine, First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China. Email: zhoucanquan@hotmail.com.
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
SB  - IM
MH  - Adult
MH  - Aneuploidy
MH  - Biopsy
MH  - Blastocyst
MH  - Chromosome Disorders/*diagnosis
MH  - *Comparative Genomic Hybridization
MH  - Embryo Culture Techniques
MH  - Embryo Transfer
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Preimplantation Diagnosis/*methods
MH  - *Translocation, Genetic
EDAT- 2014/05/14 06:00
MHDA- 2014/12/23 06:00
CRDT- 2014/05/14 06:00
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2014/12/23 06:00 [medline]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2014 Mar;49(3):193-8.