PMID- 24820470
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20211203
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 8
IP  - 5
DP  - 2014 May 14
TI  - Diagnosis of Chlamydia trachomatis in patients with reactive arthritis and 
      undifferentiated spondyloarthropathy.
PG  - 648-54
LID - 10.3855/jidc.3644 [doi]
AB  - INTRODUCTION: There is a paucity of information on the frequency of Chlamydia 
      trachomatis-induced reactive arthritis (ReA) and undifferentiated 
      spondyloarthropathy (uSpA) in India. In this study, arthritic patients suffering 
      from ReA, uSpA, and rheumatoid arthritis (RA) were screened to investigate the 
      presence of C. trachomatis infection in the synovial fluid (SF) or serum by 
      molecular and non-molecular methods. METHODOLOGY: A total of 76 arthritic 
      patients with ReA (n = 16) and uSpA (n = 22) composed the study group while those 
      with RA (n = 38) served as controls. The detection of C. trachomatis DNA was done 
      by semi-nested PCR (snPCR) and nested PCR (nPCR) targeting two different genes of 
      C. trachomatis, namely major outer membrane protein and plasmid, respectively. 
      The presence of serum or SF immunoglobulin IgG and IgA antibodies against C. 
      trachomatis was studied by commercial enzyme-linked immunosorbent assay kits. 
      RESULTS: The SF from 9 of 38 (23.6%) patients (5 with ReA and 4 with uSpA) was 
      positive for at least one C. trachomatis DNA by snPCR or nPCR in comparison to RA 
      (1/38 [2.6%]; p value < 0.05). There was no correlation between the snPCR or nPCR 
      and the serological results of patients with ReA or uSpA. CONCLUSIONS: As 
      molecular diagnostic techniques established intra-articular C. trachomatis 
      infection among this group of seronegative spondyloarthropathies in India, these 
      findings should be viewed with concern, and snPCR or nPCR should be considered 
      for a more reliable diagnosis.
FAU - Kumar, Praveen
AU  - Kumar P
AD  - National Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, 
      India. pilluigib@gmail.com.
FAU - Bhakuni, Darshan Singh
AU  - Bhakuni DS
FAU - Rastogi, Sangita
AU  - Rastogi S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Bacterial/blood
MH  - Arthritis, Reactive/*etiology/microbiology
MH  - Chlamydia Infections/complications/*diagnosis
MH  - Chlamydia trachomatis/*isolation & purification
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Female
MH  - Humans
MH  - Immunoglobulin A/blood
MH  - Immunoglobulin G/blood
MH  - India
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Prohibitins
MH  - Serum/microbiology
MH  - Spondylarthropathies/*etiology/microbiology
MH  - Synovial Fluid/microbiology
MH  - Young Adult
EDAT- 2014/05/14 06:00
MHDA- 2015/01/06 06:00
CRDT- 2014/05/14 06:00
PHST- 2013/04/08 00:00 [received]
PHST- 2013/08/19 00:00 [accepted]
PHST- 2013/08/14 00:00 [revised]
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - 10.3855/jidc.3644 [doi]
PST - epublish
SO  - J Infect Dev Ctries. 2014 May 14;8(5):648-54. doi: 10.3855/jidc.3644.