PMID- 24820628
OWN - NLM
STAT- MEDLINE
DCOM- 20150126
LR  - 20140609
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 15
IP  - 6
DP  - 2014 Jun 9
TI  - Poly(N-vinylpyrrolidinone) microgels: preparation, biocompatibility, and 
      potential application as drug carriers.
PG  - 2285-93
LID - 10.1021/bm5004493 [doi]
AB  - The biocompatible poly(N-vinylpyrrolidinone) (PNVP) microgels were synthesized 
      via surfactant free emulsion polymerization with N-vinylpyrrolidinone (NVP) as 
      the monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker at 60 
       degrees C. The obtained PNVP microgels are spherical in shape with hydrodynamic diameter 
      of approximately 200 nm and narrow size distribution. The PNVP microgels show 
      rough surfaces due to the different reaction rates of monomer NVP and 
      cross-linker EGDMA. The obtained PNVP microgels could well disperse in 
      phosphate-buffered saline (PBS) solution with long-term stability, which make 
      them candidates for bioapplications. The results of 
      3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) tests 
      indicated that the PNVP microgels are biocompatible with low toxicity even at a 
      concentration of 1000 mug/mL. By labeling the PNVP microgels with fluorescein 
      comonomer, it was demonstrated that the PNVP microgels could be uptaken by human 
      embryonic kidney 293 (HEK-293) cells. The experimental results indicated that the 
      release of isoniazid (INH, the hydrophilic model drug) could be well described by 
      a Fickian release, whereas the microgels exhibited burst release for 
      5-fluorouracil (5-fu, the hydrophobic model drug).
FAU - Yang, Qing
AU  - Yang Q
AD  - MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department 
      of Polymer Science & Engineering, Zhejiang University , Hangzhou 310027, China.
FAU - Wang, Kai
AU  - Wang K
FAU - Nie, Jingjing
AU  - Nie J
FAU - Du, Binyang
AU  - Du B
FAU - Tang, Guping
AU  - Tang G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140521
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Biocompatible Materials)
RN  - 0 (Drug Carriers)
RN  - 0 (Gels)
RN  - 0 (Polyvinyls)
RN  - 0 (Pyrrolidines)
RN  - 0 (poly(N-vinylpyrrolidine))
SB  - IM
MH  - Biocompatible Materials/administration & dosage/*chemistry
MH  - Cell Survival/drug effects/physiology
MH  - Drug Carriers/administration & dosage/*chemistry
MH  - Gels/administration & dosage/*chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - MCF-7 Cells
MH  - Polyvinyls/administration & dosage/*chemistry
MH  - Pyrrolidines/administration & dosage/*chemistry
EDAT- 2014/05/14 06:00
MHDA- 2015/01/27 06:00
CRDT- 2014/05/14 06:00
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2015/01/27 06:00 [medline]
AID - 10.1021/bm5004493 [doi]
PST - ppublish
SO  - Biomacromolecules. 2014 Jun 9;15(6):2285-93. doi: 10.1021/bm5004493. Epub 2014 
      May 21.