PMID- 24820782
OWN - NLM
STAT- MEDLINE
DCOM- 20150528
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 49
IP  - 3
DP  - 2014 Mar
TI  - [Porphyromonas gingivalis infection enhanced the adhesion of monocytes to 
      endothelial].
PG  - 151-4
AB  - OBJECTIVE: To observe if Porphyromonas gingivalis (Pg) infection could enhance 
      the adhesion of human monocytic cell line (THP-1) to human umbilical vein 
      endothelial cells (HUVEC). METHODS: PgATCC33277 was cultured in anaerobic jar, 
      and THP-1 was infected with various concentrations of PgATCC33277 at multiplicity 
      of infection (MOI) of 1: 100 for 8 and 24 hours, respectively. After removal of 
      the free Pg, THP-1 cells were cocultured with HUVEC for 1 hour to observe the 
      adhesion of THP-1 to HUVEC.HUVEC with adhesive THP-1 cells were co-cultured for 
      additional 23 hours. The medium and cells were separately collected. The 
      expression of related chemotactic cytokine[monocyte chemotactic protein 1(MCP-1) 
      and interleukin 8(IL-8)] and intercellular adhesion molecule-1(ICAM-1) were 
      detected with enzyme-linked immunosorbent assay. RESULTS: The adhesion of THP-1 
      to HUVEC was enhanced (13.8%-35.2%, P = 0.006) and the expression of ICAM-1 of 
      HUVEC was increased from (132.5 +/- 7.7) to (164.9 +/- 9.1) ng/L (P = 0.005) after 
      infection for 24 hours by Pg. Both of the secreted MCP-1 and IL-8 elevated after 
      infection of Pg for 24 hours from (183.2 +/- 3.1) to (221.0 +/- 4.9) ng/L (P = 0.012) 
      and from (587.2 +/- 5.1) to (787.2 +/- 10.3) ng/L (P = 0.002), respectively. 
      CONCLUSIONS: Pg could enhance the adhesion of monocytes to endothelial cells and 
      stimulate the inflammation, suggesting that Pg infection may be one of the risk 
      factors in promoting the development of atherosclerosis.
FAU - Zhang, Caixia
AU  - Zhang C
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
FAU - Song, Jie
AU  - Song J
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
FAU - Li, Jingjing
AU  - Li J
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
FAU - Gao, Xin
AU  - Gao X
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
FAU - Sun, Weibin
AU  - Sun W
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China.
FAU - Li, Kuanyu
AU  - Li K
AD  - Department of Cell Biology and Genetics, Medical School of Nanjing University, 
      Jiangsu Key Laboratory of Molecular Medicine, Nanjing 210093, China. Email: 
      likuanyu@nju.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Bacteroidaceae Infections/*metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Coculture Techniques
MH  - Endothelial Cells
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Inflammation
MH  - Intercellular Adhesion Molecule-1/*biosynthesis
MH  - Interleukin-8/*biosynthesis
MH  - Monocytes
MH  - Porphyromonas gingivalis/*pathogenicity
MH  - Umbilical Veins
EDAT- 2014/05/14 06:00
MHDA- 2015/05/29 06:00
CRDT- 2014/05/14 06:00
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2015/05/29 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2014 Mar;49(3):151-4.