PMID- 24821053
OWN - NLM
STAT- MEDLINE
DCOM- 20150814
LR  - 20191210
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 139
IP  - 13
DP  - 2014 Jul 7
TI  - Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe.
PG  - 3347-51
LID - 10.1039/c4an00253a [doi]
AB  - The development of simple and low-cost approaches to the detection of 
      immunoglobulin E (IgE) would provide a method for the early diagnosis and 
      prevention of atopic diseases. The current methods of detection are generally 
      tedious, multi-step processes and are limited by the high cost of the labeled 
      proteins. We describe here a label-free structure-switching colorimetric method 
      for the simple measurement of IgE using DNA pseudoknot probes and gold 
      nanoparticles. In the absence of a target the IgE aptamer probe adopts a 
      pseudoknot conformation that dissociates a capture probe from the unmodified gold 
      nanoparticles. However, when IgE binds to the aptamer probe, the pseudoknot is 
      resolved, leading to a favorable hybridization between the 3' terminal loop of 
      the aptamer probe and the capture probe; this induces the aggregation of the gold 
      nanoparticles. As a result, the colorimetric IgE sensor using this 
      structure-switching mechanism is sensitive, specific and convenient, and the 
      assay works even when challenged with complicated biological matrixes such as 
      vaginal fluids. The proposed method is expected to be of great clinical value for 
      IgE detection and could be used, after appropriate design, for sensing 
      applications of other structured aptamers.
FAU - Chang, Chia-Chen
AU  - Chang CC
AD  - Institute of Biomedical Engineering, National Taiwan University, Taipei 106, 
      Taiwan, ROC. cwlinx@ntu.edu.tw.
FAU - Chen, Chen-Yu
AU  - Chen CY
FAU - Zhao, Xihong
AU  - Zhao X
FAU - Wu, Tzu-Heng
AU  - Wu TH
FAU - Wei, Shih-Chung
AU  - Wei SC
FAU - Lin, Chii-Wann
AU  - Lin CW
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Base Sequence
MH  - Colorimetry/*methods
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Limit of Detection
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
EDAT- 2014/05/14 06:00
MHDA- 2015/08/15 06:00
CRDT- 2014/05/14 06:00
PHST- 2014/05/14 06:00 [entrez]
PHST- 2014/05/14 06:00 [pubmed]
PHST- 2015/08/15 06:00 [medline]
AID - 10.1039/c4an00253a [doi]
PST - ppublish
SO  - Analyst. 2014 Jul 7;139(13):3347-51. doi: 10.1039/c4an00253a.