PMID- 24822455
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 32
IP  - 2
DP  - 2014 Feb
TI  - [Determination of N-carbamyl-L-glutamic acid in feedstuff by high performance 
      liquid chromatography coupled with electrospray ionization tandem mass 
      spectrometry].
PG  - 184-8
AB  - A method was developed for the determination of N-carbamyl-L-glutamic acid (NCG) 
      in feedstuff by high performance liquid chromatography coupled with electrospray 
      ionization tandem mass spectrometry (HPLC-ESI-MS/MS). The feedstuff samples were 
      extracted with methanol, cleaned-up by a ProElut mixed-mode of strong anion 
      exchange inverse column (PXA). Then the samples were separated with HPLC and 
      detected with MS/MS in multiple reaction monitoring (MRM) mode via positive 
      electrospray ionization (ESI+). The fragment ions of m/z 148.0 and m/z 84.0 were 
      used for qualitative analysis, and m/z 130.0 was selected for quantitative 
      analysis. The limit of detection for NCG was 24 microg/kg (S/N > 3), and the 
      limit of quantification was 80 microg/kg (S/N > 10). The standard calibration 
      curve was linear over the range of 20-1,000 microg/L, and the correlation 
      coefficient was 0. 999 9. The recoveries of NCG in feedstuff were 104.0%, 103.5%, 
      95.3%, at the three spiked levels of 80, 200, 500 mg/kg with the relative 
      standard deviations (RSDs) of 7.5%, 6.3% and 5.8%. The results showed that the 
      method is simple, fast, highly sensitive, specific and with good purification 
      effect. It can be applied to the analysis and determination of NCG in feedstuff.
FAU - Tang, Shengyun
AU  - Tang S
FAU - Wang, Yuanxing
AU  - Wang Y
FAU - Wen, Pingwei
AU  - Wen P
FAU - Xin, Zhen
AU  - Xin Z
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Glutamic Acid/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
EDAT- 2014/05/16 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/05/15 06:00
PHST- 2014/05/15 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.3724/sp.j.1123.2013.09027 [doi]
PST - ppublish
SO  - Se Pu. 2014 Feb;32(2):184-8. doi: 10.3724/sp.j.1123.2013.09027.