PMID- 24823336
OWN - NLM
STAT- MEDLINE
DCOM- 20150519
LR  - 20180302
IS  - 1559-0488 (Electronic)
IS  - 1559-047X (Linking)
VI  - 35
IP  - 5
DP  - 2014 Sep-Oct
TI  - Optimization of a polyurethane dermal matrix and experience with a polymer-based 
      cultured composite skin.
PG  - 437-48
LID - 10.1097/BCR.0000000000000061 [doi]
AB  - The aims were to (1) describe the in vivo studies leading to an optimized model 
      of the biodegradable temporizing matrix (BTM), (2) describe our efforts in 
      effecting closure over this optimized matrix after integration with a cultured 
      composite skin (CCS), and (3) reexamine the ability of the CCS to definitively 
      close fresh wounds (without BTM). Foam scaffolds of biodegradable polyurethane 
      were created to allow in vivo tissue ingrowth or in vitro co-culture. Using the 
      porcine surgical model, multiple BTM optimization studies took place before the 
      BTM-CCS main study was conducted. For the CCS study, optimized sealed 2 mm 
      matrices were implanted into 6-mm deep, 8 x 8 cm wounds (three per pig) and 
      allowed to integrate for 21 days, whereas collected blood and harvested skin 
      tissue were used to prepare autologous composite skins in similar (unsealed) 1 mm 
      matrices. These were then applied at day 21 either over the integrated BTMs or 
      into a freshly created fourth wound. All of the optimized matrices integrated 
      fully, without loss, and were found to resist wound contraction effectively until 
      the composites were ready for application at day 21. The composites demonstrated 
      the ability to generate a bilayer repair with robust epidermis anchored by a 
      basement membrane visible from day 7 after application. The final optimized 
      sealed BTM delaminates easily to produce a clean, temporized wound bed and will 
      be used in the upcoming burn clinical trial. Although the CCS is a magnitude away 
      from human trials, it is still capable of generating a bilayer repair in both 
      BTM-integrated and fresh wounds (onto fat), and with further refinement and 
      optimization of foam structure, seeding densities, and timing, consistent success 
      should be possible.
FAU - Dearman, Bronwyn L
AU  - Dearman BL
AD  - From the *Skin Engineering Laboratory and daggerAdult Burn Centre, Royal Adelaide 
      Hospital, Adelaide, South Australia, Australia.
FAU - Li, Amy
AU  - Li A
FAU - Greenwood, John E
AU  - Greenwood JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Burn Care Res
JT  - Journal of burn care & research : official publication of the American Burn 
      Association
JID - 101262774
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 0 (Polyurethanes)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Biopsy
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Polymers/*pharmacology
MH  - Polyurethanes/*pharmacology
MH  - Skin Transplantation/*methods
MH  - *Skin, Artificial
MH  - Swine
MH  - Water Loss, Insensible
MH  - Wound Healing/*physiology
EDAT- 2014/05/16 06:00
MHDA- 2015/05/20 06:00
CRDT- 2014/05/15 06:00
PHST- 2014/05/15 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
AID - 10.1097/BCR.0000000000000061 [doi]
PST - ppublish
SO  - J Burn Care Res. 2014 Sep-Oct;35(5):437-48. doi: 10.1097/BCR.0000000000000061.