PMID- 2482339
OWN - NLM
STAT- MEDLINE
DCOM- 19900314
LR  - 20190828
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 29
IP  - 3
DP  - 1989 Nov
TI  - Human immunodeficiency virus type 1 p24 production and antigenic variation in 
      tissue culture of isolates with various growth characteristics.
PG  - 170-5
AB  - Two monoclonal antibodies (Mabs) reacting with different epitopes of the human 
      immunodeficiency virus type 1 core protein p24 (HIV p24) were used either singly 
      or in combination as tracers in enzyme-linked immunosorbent assays. The culture 
      supernatant of 215 samples of peripheral blood mononuclear cells from 112 
      patients were measured for HIV p24 and reverse transcriptase (RT) activity during 
      cultivation. One hundred forty-one cultures were positive for HIV p24 and 122 for 
      RT after 32 days of cultivation. After 8-9 days, HIV p24 was detected in 50.4% 
      and RT in 27.8% of the cultures later judged as HIV positive. Two patients seemed 
      to have substrains of HIV-1 not reactive with one of the Mabs.
FAU - Sundqvist, V A
AU  - Sundqvist VA
AD  - Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
FAU - Albert, J
AU  - Albert J
FAU - Ohlsson, E
AU  - Ohlsson E
FAU - Hinkula, J
AU  - Hinkula J
FAU - Fenyo, E M
AU  - Fenyo EM
FAU - Wahren, B
AU  - Wahren B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Antigens)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (Viral Core Proteins)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/immunology
MH  - Antibodies, Monoclonal
MH  - *Antigenic Variation
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Products, gag/*biosynthesis
MH  - HIV Antigens/*analysis
MH  - HIV Core Protein p24
MH  - HIV-1/*growth & development/immunology/isolation & purification
MH  - Humans
MH  - Kinetics
MH  - RNA-Directed DNA Polymerase/blood
MH  - Viral Core Proteins/*biosynthesis
EDAT- 1989/11/01 00:00
MHDA- 1989/11/01 00:01
CRDT- 1989/11/01 00:00
PHST- 1989/11/01 00:00 [pubmed]
PHST- 1989/11/01 00:01 [medline]
PHST- 1989/11/01 00:00 [entrez]
AID - 10.1002/jmv.1890290305 [doi]
PST - ppublish
SO  - J Med Virol. 1989 Nov;29(3):170-5. doi: 10.1002/jmv.1890290305.