PMID- 24824254
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20140514
IS  - 2542-5641 (Electronic)
IS  - 0366-6999 (Linking)
VI  - 127
IP  - 10
DP  - 2014
TI  - Urotensin II promotes monocyte chemoattractant protein-1 expression in aortic 
      adventitial fibroblasts of rat.
PG  - 1907-12
AB  - BACKGROUND: Urotensin II (UII), a potent vasoconstrictive peptide, is able to 
      stimulate phenotypic differentiation of adventitial fibroblasts. This study aimed 
      to determine the effect of UII on monocyte chemoattractant protein-1 (MCP-1) 
      expression in rat aortic adventitial fibroblasts, so as to explore possible 
      mechanisms in the development of vascular inflammation. METHODS: Growth-arrested 
      adventitial fibroblasts were incubated in serum-free medium with UII 
      (10(-10)-10(-7) mol/L) and inhibitors of signal transduction pathways for 1 to 24 
      hours. MCP-1 mRNA and protein expression and secretion were determined by RT-PCR, 
      Western blotting analysis and enzyme-linked immunosorbent assay (ELISA), 
      respectively. RESULTS: UII dose- and time-dependently promoted MCP-1 mRNA and 
      protein expression and secretion in cells, with maximal effect at 10(-8) mol/L at 
      3 hours for mRNA expression, 24 hours for protein expression in the cells, and 12 
      hours for protein secretion from the cells. Furthermore, the UII effects were 
      significantly inhibited by treatment with its receptor antagonist SB710411, Rho 
      kinase inhibitor Y27632, protein kinase C (PKC) inhibitor H7, mitogen-activated 
      protein kinase inhibitor PD98059, calcineurin inhibitor cyclosporine A, and the 
      Ca(2+)channel blocker nicardipine. CONCLUSION: UII may stimulate MCP-1 expression 
      in rat aortic adventitial fibroblasts through its receptor and Rho kinase, PKC, 
      mitogen-activated protein kinase, calcineurin and Ca(2+) channel signal 
      transduction, thus contributing to adventitial inflammation.
FAU - Zhang, Yonggang
AU  - Zhang Y
AD  - Department of Cardiology, Second Affiliated Hospital, Shantou University Medical 
      College, Shantou, Guangdong 515041, China; Cardiovascular Laboratory, Centre for 
      Translational Medicine, Shantou University Medical College, Shantou, Guangdong 
      515041, China. Email: zhangyg8686@hotmail.com.
FAU - Bao, Shilin
AU  - Bao S
AD  - Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou 
      University Medical College, Shantou, Guangdong 515041, China.
FAU - Kuang, Zejian
AU  - Kuang Z
AD  - Department of Cardiology, Second Affiliated Hospital, Shantou University Medical 
      College, Shantou, Guangdong 515041, China.
FAU - Ma, Yanjun
AU  - Ma Y
AD  - Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou 
      University Medical College, Shantou, Guangdong 515041, China.
FAU - Hu, Yanchao
AU  - Hu Y
AD  - Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou 
      University Medical College, Shantou, Guangdong 515041, China.
FAU - Mao, Yanyan
AU  - Mao Y
AD  - Department of Cardiovascular Diseases, First Affiliated Hospital, Shantou 
      University Medical College, Shantou, Guangdong 515041, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Urotensins)
RN  - 9047-55-6 (urotensin II)
SB  - IM
MH  - Adventitia/*cytology
MH  - Animals
MH  - Aorta/*cytology
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Fibroblasts/*drug effects/*metabolism
MH  - Male
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Urotensins/*pharmacology
EDAT- 2014/05/16 06:00
MHDA- 2015/04/07 06:00
CRDT- 2014/05/15 06:00
PHST- 2014/05/15 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
PST - ppublish
SO  - Chin Med J (Engl). 2014;127(10):1907-12.