PMID- 24824862 OWN - NLM STAT- MEDLINE DCOM- 20150512 LR - 20220409 IS - 1873-4847 (Electronic) IS - 0955-2863 (Linking) VI - 25 IP - 8 DP - 2014 Aug TI - Lactoferrin up-regulates intestinal gene expression of brain-derived neurotrophic factors BDNF, UCHL1 and alkaline phosphatase activity to alleviate early weaning diarrhea in postnatal piglets. PG - 834-42 LID - S0955-2863(14)00078-3 [pii] LID - 10.1016/j.jnutbio.2014.03.015 [doi] AB - The molecular mechanisms underlying how dietary lactoferrin (Lf) impacts gut development and maturation and protects against early weaning diarrhea are not well understood. In this study, we supplemented postnatal piglets with an Lf at a dose level of 155 and 285 mg/kg/day from 3 to 38 days following birth. Our findings show that the high dose of Lf up-regulated messenger RNA expression levels of genes encoding brain-derived neurotrophic factor (BDNF) and ubiquitin carboxy-terminal hydrolase L1 (ubiquitin thiolesterase (UCHL1) and, to a lesser extent, glial cell line-derived neurotrophic factor, in the duodenum (P<.05). Piglets in the high and low Lf group had 30% and 7% larger jejunal crypts compared with the control group (P<.05). Escherichia coli 16S rRNA copy number per gram of ascending colon contents was significantly reduced (P=.001), while the copy number of Bifidobacteria and Lactobacillus spp. was not affected. In addition, Lf increased intestinal alkaline phosphatase activity (P<.05) and delayed the onset of food transitional diarrhea, reducing its frequency and duration (P<.05). The incidence of diarrhea in the high and low Lf groups was decreased 54% and 15%, respectively, compared with the control group (P=.035). In summary, these findings provide new evidence that dietary Lf supplementation up-regulated gene expression of BDNF and UCHL1, decreased the colon microbiota of E. coli, improved gut maturation and reduced early weaning diarrhea in piglets. The molecular basis underlying these findings suggests that Lf may enhance gut development and immune function by providing new insight into the gut-brain-microbe axis that has not been previously reported. CI - Copyright (c) 2014 Elsevier Inc. All rights reserved. FAU - Yang, Changwei AU - Yang C AD - School of Medicine, Xiamen University, Xiamen, 361102, China. FAU - Zhu, Xi AU - Zhu X AD - School of Medicine, Xiamen University, Xiamen, 361102, China. FAU - Liu, Ni AU - Liu N AD - School of Medicine, Xiamen University, Xiamen, 361102, China. FAU - Chen, Yue AU - Chen Y AD - School of Medicine, Xiamen University, Xiamen, 361102, China. FAU - Gan, Hexia AU - Gan H AD - School of Medicine, Xiamen University, Xiamen, 361102, China. FAU - Troy, Frederic A 2nd AU - Troy FA 2nd AD - School of Medicine, Xiamen University, Xiamen, 361102, China; Department of Biochemistry and Molecular Medicine, University of California School of Medicine, Davis, CA 95616, USA. FAU - Wang, Bing AU - Wang B AD - School of Medicine, Xiamen University, Xiamen, 361102, China; School of Animal and Veterinary Science, Charles Sturt University NSW 2678, Australia. Electronic address: biwang@csu.edu.au. LA - eng GR - GM5503/GM/NIGMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20140413 PL - United States TA - J Nutr Biochem JT - The Journal of nutritional biochemistry JID - 9010081 RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 3.1.3.1 (Alkaline Phosphatase) RN - EC 3.2.1.108 (Lactase) RN - EC 3.4.19.12 (Ubiquitin Thiolesterase) RN - EC 3.4.21.- (Lactoferrin) SB - IM MH - Alkaline Phosphatase/*genetics MH - Animals MH - Body Weight/drug effects MH - Brain-Derived Neurotrophic Factor/*genetics MH - Colon/drug effects/microbiology MH - Diarrhea/*diet therapy/genetics/prevention & control MH - Dietary Supplements MH - Duodenum/drug effects MH - Gene Expression Regulation/drug effects MH - Intestinal Mucosa/drug effects/enzymology MH - Intestines/drug effects MH - Jejunum/drug effects/enzymology MH - Lactase/metabolism MH - Lactoferrin/*pharmacology MH - Male MH - Sus scrofa MH - Ubiquitin Thiolesterase/*genetics MH - Up-Regulation/drug effects MH - Weaning OTO - NOTNLM OT - BDNF OT - Colonic flora OT - Gut maturation OT - IAP OT - Lactoferrin OT - Neurotrophins OT - UCHL1 EDAT- 2014/05/16 06:00 MHDA- 2015/05/13 06:00 CRDT- 2014/05/15 06:00 PHST- 2013/10/22 00:00 [received] PHST- 2014/03/14 00:00 [revised] PHST- 2014/03/17 00:00 [accepted] PHST- 2014/05/15 06:00 [entrez] PHST- 2014/05/16 06:00 [pubmed] PHST- 2015/05/13 06:00 [medline] AID - S0955-2863(14)00078-3 [pii] AID - 10.1016/j.jnutbio.2014.03.015 [doi] PST - ppublish SO - J Nutr Biochem. 2014 Aug;25(8):834-42. doi: 10.1016/j.jnutbio.2014.03.015. Epub 2014 Apr 13.