PMID- 24825422 OWN - NLM STAT- MEDLINE DCOM- 20140829 LR - 20230810 IS - 1556-5653 (Electronic) IS - 0015-0282 (Print) IS - 0015-0282 (Linking) VI - 102 IP - 1 DP - 2014 Jul TI - Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation. PG - 135-142.e6 LID - S0015-0282(14)00353-7 [pii] LID - 10.1016/j.fertnstert.2014.04.008 [doi] AB - OBJECTIVE: To use trophoblast cells accumulating in the endocervical canal at the beginning of pregnancy for noninvasive prenatal testing. DESIGN: Prospective, double-blinded test for fetal gender. SETTING: Academic medical center. PATIENT(S): Fifty-six women with singleton pregnancies at gestational age 5-20 weeks. INTERVENTION(S): Isolation of fetal cells from resident maternal cells in endocervical specimens using anti-human leukocyte antigen G coupled to magnetic nanoparticles; cell phenotyping immunofluorescently with a panel of trophoblast subtype-specific proteins; DNA integrity assessment with terminal dUTP nick-end labeling (TUNEL); and polymerase chain reaction (PCR) and fluorescent in situ hybridization (FISH) to detect sex chromosomes in individual cells. MAIN OUTCOME MEASURE(S): Trophoblast phenotype, TUNEL index, and percentage male cells. RESULT(S): The women were given a routine Papanicolaou test; fetal genders were verified from medical records. Recovery after immunomagnetic isolation averaged 746+/-59 cells across gestational age, with 99% expressing chorionic gonadotropin, whereas the depleted cell fraction expressed none. The isolated cells had an extravillous trophoblast phenotype and intact nuclear DNA (>95%). Fetal gender was determined in 20 specimens without error by PCR. The FISH analysis of isolated cells from male specimens validated their fetal origin. CONCLUSION(S): Noninvasive prenatal testing is feasible beginning at a gestational age of 5 weeks. CI - Copyright (c) 2014. Published by Elsevier Inc. FAU - Bolnick, Jay M AU - Bolnick JM AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Kilburn, Brian A AU - Kilburn BA AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Bajpayee, Swati AU - Bajpayee S AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Reddy, Nitya AU - Reddy N AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Jeelani, Roohi AU - Jeelani R AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Crone, Barbara AU - Crone B AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Simmerman, Neil AU - Simmerman N AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Singh, Manivinder AU - Singh M AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan. FAU - Diamond, Michael P AU - Diamond MP AD - Department of Obstetrics and Gynecology, Georgia Regents University, Augusta, Georgia. FAU - Armant, D Randall AU - Armant DR AD - Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan; Anatomy and Cell Biology, C.S. Mott Center for Human Growth and Development, Wayne State University, Detroit, Michigan; Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland. Electronic address: d.armant@wayne.edu. LA - eng GR - R21 HD071408/HD/NICHD NIH HHS/United States GR - ImNIH/Intramural NIH HHS/United States GR - HD071408/HD/NICHD NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't DEP - 20140510 PL - United States TA - Fertil Steril JT - Fertility and sterility JID - 0372772 RN - 0 (Biomarkers) RN - 0 (Chorionic Gonadotropin, beta Subunit, Human) SB - IM MH - Academic Medical Centers MH - Biomarkers/metabolism MH - Cell Lineage MH - Cells, Cultured MH - Cervix Uteri/*metabolism MH - Chorionic Gonadotropin, beta Subunit, Human/metabolism MH - Chromosomes, Human, X MH - Chromosomes, Human, Y MH - DNA Fragmentation MH - Double-Blind Method MH - Female MH - Genetic Testing MH - Genotype MH - Gestational Age MH - Humans MH - *Immunomagnetic Separation MH - In Situ Hybridization, Fluorescence MH - In Situ Nick-End Labeling MH - Male MH - Phenotype MH - Polymerase Chain Reaction MH - Predictive Value of Tests MH - Pregnancy MH - Prenatal Diagnosis/*methods MH - Prospective Studies MH - Reproducibility of Results MH - *Sex Determination Analysis MH - Trophoblasts/*metabolism PMC - PMC10411519 MID - NIHMS1920999 OTO - NOTNLM OT - Endocervical canal OT - fetal gender OT - prenatal testing OT - single-cell analysis OT - trophoblast EDAT- 2014/05/16 06:00 MHDA- 2014/08/30 06:00 PMCR- 2023/08/09 CRDT- 2014/05/15 06:00 PHST- 2014/01/26 00:00 [received] PHST- 2014/04/06 00:00 [revised] PHST- 2014/04/07 00:00 [accepted] PHST- 2014/05/15 06:00 [entrez] PHST- 2014/05/16 06:00 [pubmed] PHST- 2014/08/30 06:00 [medline] PHST- 2023/08/09 00:00 [pmc-release] AID - S0015-0282(14)00353-7 [pii] AID - 10.1016/j.fertnstert.2014.04.008 [doi] PST - ppublish SO - Fertil Steril. 2014 Jul;102(1):135-142.e6. doi: 10.1016/j.fertnstert.2014.04.008. Epub 2014 May 10.