PMID- 24825569
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20211021
IS  - 1689-1392 (Electronic)
IS  - 1425-8153 (Print)
IS  - 1425-8153 (Linking)
VI  - 19
IP  - 2
DP  - 2014 Jun
TI  - Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for 
      skin tissue engineering.
PG  - 297-314
LID - 10.2478/s11658-014-0197-1 [doi]
AB  - Degradable aliphatic polyesters such as polylactides, polyglycolides and their 
      copolymers are used in several biomedical and pharmaceutical applications. We 
      analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the 
      adhesion, proliferation, motility and differentiation of primary human skin 
      keratinocytes and fibroblasts in the context of their potential use as cell 
      carriers for skin tissue engineering. We did not observe visible differences in 
      the morphology, focal contact appearance, or actin cytoskeleton organization of 
      skin cells cultured on PLGA films compared to those cultured under control 
      conditions. Moreover, we did not detect biologically significant differences in 
      proliferative activity, migration parameters, level of differentiation, or 
      expression of vinculin when the cells were cultured on PLGA films and tissue 
      culture polystyrene. Our results indicate that PLGA films do not affect the basic 
      functions of primary human skin keratinocytes and fibroblasts and thus show 
      acceptable biocompatibility in vitro, paving the way for their use as 
      biomaterials for skin tissue engineering.
FAU - Zuber, Aleksandra
AU  - Zuber A
AD  - Laboratory of Cell & Tissue Engineering, Department of Cell Biology, Faculty of 
      Biochemistry, Biophysics and Biotechnology and Malopolska Centre of 
      Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
FAU - Borowczyk, Julia
AU  - Borowczyk J
FAU - Zimolag, Eliza
AU  - Zimolag E
FAU - Krok, Malgorzata
AU  - Krok M
FAU - Madeja, Zbigniew
AU  - Madeja Z
FAU - Pamula, Elzbieta
AU  - Pamula E
FAU - Drukala, Justyna
AU  - Drukala J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140513
PL  - England
TA  - Cell Mol Biol Lett
JT  - Cellular & molecular biology letters
JID - 9607427
RN  - 0 (Biocompatible Materials)
RN  - 125361-02-6 (Vinculin)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Actin Cytoskeleton/drug effects
MH  - Biocompatible Materials/chemical synthesis/*chemistry/pharmacology
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Fibroblasts/cytology/metabolism
MH  - Humans
MH  - Keratinocytes/cytology/metabolism
MH  - Lactic Acid/chemical synthesis/*chemistry/pharmacology
MH  - Polyglycolic Acid/chemical synthesis/*chemistry/pharmacology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Regeneration/drug effects
MH  - Skin/metabolism
MH  - Surface Properties
MH  - *Tissue Engineering
MH  - Vinculin/metabolism
PMC - PMC6275924
EDAT- 2014/05/16 06:00
MHDA- 2015/02/20 06:00
PMCR- 2014/05/13
CRDT- 2014/05/15 06:00
PHST- 2013/12/08 00:00 [received]
PHST- 2014/05/05 00:00 [accepted]
PHST- 2014/05/15 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
PHST- 2014/05/13 00:00 [pmc-release]
AID - 197 [pii]
AID - 10.2478/s11658-014-0197-1 [doi]
PST - ppublish
SO  - Cell Mol Biol Lett. 2014 Jun;19(2):297-314. doi: 10.2478/s11658-014-0197-1. Epub 
      2014 May 13.