PMID- 24829249
OWN - NLM
STAT- MEDLINE
DCOM- 20150213
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 52
IP  - 7
DP  - 2014 Jul
TI  - Detection of Herpesviridae in whole blood by multiplex PCR DNA-based microarray 
      analysis after hematopoietic stem cell transplantation.
PG  - 2552-6
LID - 10.1128/JCM.00061-14 [doi]
AB  - Viral infections are important causes of morbidity and mortality in patients 
      after hematopoietic stem cell transplantation. The monitoring by PCR of 
      Herpesviridae loads in blood samples has become a critical part of posttransplant 
      follow-up, representing mounting costs for the laboratory. In this study, we 
      assessed the clinical performance of the multiplex PCR DNA microarray Clart 
      Entherpex kit for detection of cytomegalovirus (CMV), Epstein-Barr virus (EBV), 
      and human herpesvirus 6 (HHV-6) as a screening test for virological follow-up. 
      Two hundred fifty-five blood samples from 16 transplanted patients, prospectively 
      tested by routine PCR assays, were analyzed by microarray. Routine PCR detected 
      single or multiple viruses in 42% and 10% of the samples, respectively. 
      Microarray detected single or multiple viruses in 34% and 18% of the samples, 
      respectively. Microarray results correlated well with CMV and EBV detections by 
      routine PCR (kappa tests = 0.79 and 0.78, respectively), whereas a weak 
      correlation was observed with HHV-6 (0.43). HHV-7 was also detected in 48 samples 
      by microarray. In conclusion, the microarray is a reliable screening assay for a 
      posttransplant virological follow-up to detect CMV and EBV infections in blood. 
      However, positive samples must be subsequently confirmed and viral loads must be 
      quantified by PCR assays. Limitations were identified regarding HHV-6 detection. 
      Although it is promising, is easy to use as a first-line test, and allows a 
      reduction in the cost of analysis without undue delay in the reporting of the 
      final quantitative result to the clinician, some characteristics of this 
      microarray should be improved, particularly regarding quality control and the 
      targeted virus panel, such that it could then be used as a routine test.
CI  - Copyright (c) 2014, American Society for Microbiology. All Rights Reserved.
FAU - Debaugnies, France
AU  - Debaugnies F
AD  - Center for Molecular Diagnostic, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium fdebaugn@ulb.ac.be.
FAU - Busson, Laurent
AU  - Busson L
AD  - Department of Microbiology, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
FAU - Ferster, Alina
AU  - Ferster A
AD  - Department of Haematology and Oncology, Hopital Universitaire Des Enfants Reine 
      Fabiola, Brussels, Belgium.
FAU - Lewalle, Philippe
AU  - Lewalle P
AD  - Department of Haematology, Jules Bordet Institute, Brussels, Belgium.
FAU - Azzi, Nadira
AU  - Azzi N
AD  - Department of Haematology and Oncology, Hopital Universitaire Des Enfants Reine 
      Fabiola, Brussels, Belgium.
FAU - Aoun, Mickael
AU  - Aoun M
AD  - Division of Infectious Diseases, Jules Bordet Institute, Brussels, Belgium.
FAU - Verhaegen, Godelieve
AU  - Verhaegen G
AD  - Center for Molecular Diagnostic, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
FAU - Mahadeb, Bhavna
AU  - Mahadeb B
AD  - Department of Microbiology, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
FAU - de Marchin, Jerome
AU  - de Marchin J
AD  - Center for Molecular Diagnostic, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
FAU - Vandenberg, Olivier
AU  - Vandenberg O
AD  - Department of Microbiology, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
FAU - Hallin, Marie
AU  - Hallin M
AD  - Center for Molecular Diagnostic, iris-Lab, Iris-Brussels Public Hospital Network, 
      Brussels, Belgium.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Blood/*virology
MH  - Child
MH  - Child, Preschool
MH  - Cytomegalovirus/classification/genetics/*isolation & purification
MH  - Female
MH  - Hematopoietic Stem Cell Transplantation/adverse effects
MH  - Herpesviridae Infections/*diagnosis/virology
MH  - Herpesvirus 4, Human/classification/genetics/*isolation & purification
MH  - Herpesvirus 6, Human/classification/genetics/*isolation & purification
MH  - Humans
MH  - Immunocompromised Host
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Mass Screening/methods
MH  - Microarray Analysis/*methods
MH  - Molecular Diagnostic Techniques/methods
MH  - Multiplex Polymerase Chain Reaction/*methods
PMC - PMC4097728
EDAT- 2014/05/16 06:00
MHDA- 2015/02/14 06:00
PMCR- 2015/01/01
CRDT- 2014/05/16 06:00
PHST- 2014/05/16 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2015/02/14 06:00 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - JCM.00061-14 [pii]
AID - 00061-14 [pii]
AID - 10.1128/JCM.00061-14 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2014 Jul;52(7):2552-6. doi: 10.1128/JCM.00061-14. Epub 2014 May 
      14.