PMID- 24829411
OWN - NLM
STAT- MEDLINE
DCOM- 20140818
LR  - 20171116
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 192
IP  - 12
DP  - 2014 Jun 15
TI  - Antigen delivery to CD11c+CD8- dendritic cells induces protective immune 
      responses against experimental melanoma in mice in vivo.
PG  - 5830-8
LID - 10.4049/jimmunol.1300975 [doi]
AB  - Dendritic cells (DCs) are central modulators of immune responses and, therefore, 
      interesting target cells for the induction of antitumor immune responses. Ag 
      delivery to select DC subpopulations via targeting Abs to DC inhibitory receptor 
      2 (DCIR2, clone 33D1) or to DEC205 was shown to direct Ags specifically to 
      CD11c(+)CD8(-) or CD11c(+)CD8(+) DCs, respectively, in vivo. In contrast to the 
      increasing knowledge about the induction of immune responses by efficiently 
      cross-presenting CD11c(+)CD8(+) DCs, little is known about the functional role of 
      Ag-presenting CD11c(+)CD8(-) DCs with regard to the initiation of protective 
      immune responses. In this study, we demonstrate that Ag targeting to the 
      CD11c(+)CD8(-) DC subpopulation in the presence of stimulating anti-CD40 Ab and 
      TLR3 ligand polyinosinic-polycytidylic acid induces protective responses against 
      rapidly growing tumor cells in naive animals under preventive and therapeutic 
      treatment regimens in vivo. Of note, this immunization protocol induced a mixed 
      Th1/Th2-driven immune response, irrespective of which DC subpopulation initially 
      presented the Ag. Our results provide important information about the role of 
      CD11c(+)CD8(-) DCs, which have been considered to be less efficient at 
      cross-presenting Ags, in the induction of protective antitumor immune responses.
CI  - Copyright (c) 2014 by The American Association of Immunologists, Inc.
FAU - Neubert, Kirsten
AU  - Neubert K
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Lehmann, Christian H K
AU  - Lehmann CH
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Heger, Lukas
AU  - Heger L
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Baranska, Anna
AU  - Baranska A
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Staedtler, Anna Maria
AU  - Staedtler AM
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Buchholz, Veit R
AU  - Buchholz VR
AD  - Institute for Medical Microbiology, Immunology, and Hygiene, Technical University 
      of Munich (TUM), 81675 Munich, Germany;
FAU - Yamazaki, Sayuri
AU  - Yamazaki S
AD  - Department of Geriatric and Environmental Dermatology, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya 467-8601, Japan;
FAU - Heidkamp, Gordon F
AU  - Heidkamp GF
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Eissing, Nathalie
AU  - Eissing N
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany;
FAU - Zebroski, Henry
AU  - Zebroski H
AD  - Proteomics Resource Center, The Rockefeller University, New York 10065;
FAU - Nussenzweig, Michel C
AU  - Nussenzweig MC
AD  - Laboratory of Molecular Immunology, The Rockefeller University, New York 10065; 
      and.
FAU - Nimmerjahn, Falk
AU  - Nimmerjahn F
AD  - Department of Biology, Friedrich-Alexander University Erlangen-Nurnberg, 91058 
      Erlangen, Germany.
FAU - Dudziak, Diana
AU  - Dudziak D
AD  - Department of Dermatology, Laboratory of Dendritic Cell Biology, 
      Friedrich-Alexander University Erlangen-Nurnberg, University Hospital Erlangen, 
      91052 Erlangen, Germany; diana.dudziak@uk-erlangen.de.
LA  - eng
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD8 Antigens)
RN  - 0 (Interferon Inducers)
RN  - 0 (TLR3 protein, mouse)
RN  - 0 (Toll-Like Receptor 3)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Antigen Presentation/drug effects/immunology
MH  - Antigens, Neoplasm/immunology/*pharmacology
MH  - CD11c Antigen/*immunology
MH  - CD8 Antigens/*immunology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology/pathology
MH  - Interferon Inducers/pharmacology
MH  - Male
MH  - Melanoma/immunology/pathology/*therapy
MH  - Mice
MH  - Neoplasms, Experimental/immunology/pathology/*therapy
MH  - Poly I-C/pharmacology
MH  - Toll-Like Receptor 3/agonists/immunology
EDAT- 2014/05/16 06:00
MHDA- 2014/08/19 06:00
CRDT- 2014/05/16 06:00
PHST- 2014/05/16 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2014/08/19 06:00 [medline]
AID - jimmunol.1300975 [pii]
AID - 10.4049/jimmunol.1300975 [doi]
PST - ppublish
SO  - J Immunol. 2014 Jun 15;192(12):5830-8. doi: 10.4049/jimmunol.1300975. Epub 2014 
      May 14.