PMID- 24829527
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140515
LR  - 20211021
IS  - 0974-1208 (Print)
IS  - 1998-4766 (Electronic)
IS  - 1998-4766 (Linking)
VI  - 7
IP  - 1
DP  - 2014 Jan
TI  - Pre-implantation genetic screening using fluorescence in situ hybridization in 
      couples of Indian ethnicity: Is there a scope?
PG  - 25-9
LID - 10.4103/0974-1208.130812 [doi]
AB  - CONTEXT: There is a high incidence of numerical chromosomal aberration in couples 
      with repeated in vitro fertilization (IVF) failure, advanced maternal age, 
      repeated unexplained abortions, severe male factor infertility and unexplained 
      infertility. Pre-implantation genetic screening (PGS), a variant of 
      pre-implantation genetic diagnosis, screens numerical chromosomal aberrations in 
      couples with normal karyotype, experiencing poor reproductive outcome. The 
      present study includes the results of the initial pilot study on 9 couples who 
      underwent 10 PGS cycles. AIM: The aim of the present study was to evaluate the 
      beneficial effects of PGS in couples with poor reproductive outcome. SETTINGS AND 
      DESIGN: Data of initial 9 couples who underwent 10 PGS for various indications 
      was evaluated. SUBJECTS AND METHODS: Blastomere biopsy was performed on cleavage 
      stage embryos and subjected to two round fluorescence in situ hybridization 
      (FISH) testing for chromosomes 13, 18, 21, X and Y as a two-step procedure. 
      RESULTS: Six of the 9 couples (10 PGS cycles) conceived, including a twin 
      pregnancy in a couple with male factor infertility, singleton pregnancies in a 
      couple with secondary infertility, in three couples with adverse obstetric 
      outcome in earlier pregnancies and in one couple with repeated IVF failure. 
      CONCLUSION: In the absence of availability of array-comparative genomic 
      hybridization in diagnostic clinical scenario for PGS and promising results with 
      FISH based PGS as evident from the current pilot study, it is imperative to offer 
      the best available services in the present scenario for better pregnancy outcome 
      for patients.
FAU - Gada Saxena, Shailaja
AU  - Gada Saxena S
AD  - Department of Molecular Medicine, Reliance Life Sciences Pvt. Ltd., Dhirubhai 
      Ambani Life Sciences Centre, Navi Mumbai, Maharashtra, India.
FAU - Desai, Kundanbala
AU  - Desai K
AD  - Department of Molecular Medicine, Reliance Life Sciences Pvt. Ltd., Dhirubhai 
      Ambani Life Sciences Centre, Navi Mumbai, Maharashtra, India.
FAU - Shewale, Lata
AU  - Shewale L
AD  - Department of Molecular Medicine, Reliance Life Sciences Pvt. Ltd., Dhirubhai 
      Ambani Life Sciences Centre, Navi Mumbai, Maharashtra, India.
FAU - Ranjan, Prabhat
AU  - Ranjan P
AD  - Department of Molecular Medicine, Reliance Life Sciences Pvt. Ltd., Dhirubhai 
      Ambani Life Sciences Centre, Navi Mumbai, Maharashtra, India.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Hum Reprod Sci
JT  - Journal of human reproductive sciences
JID - 101473512
PMC - PMC4018794
OTO - NOTNLM
OT  - Aneuploidy testing
OT  - fluorescence in situ hybridization
OT  - genetic counseling
OT  - pre-implantation genetic diagnosis
OT  - pre-implantation genetic screening
COIS- Conflict of Interest: None declared.
EDAT- 2014/05/16 06:00
MHDA- 2014/05/16 06:01
PMCR- 2014/01/01
CRDT- 2014/05/16 06:00
PHST- 2013/06/03 00:00 [received]
PHST- 2013/09/03 00:00 [revised]
PHST- 2014/01/14 00:00 [accepted]
PHST- 2014/05/16 06:00 [entrez]
PHST- 2014/05/16 06:00 [pubmed]
PHST- 2014/05/16 06:01 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - JHRS-7-25 [pii]
AID - 10.4103/0974-1208.130812 [doi]
PST - ppublish
SO  - J Hum Reprod Sci. 2014 Jan;7(1):25-9. doi: 10.4103/0974-1208.130812.