PMID- 24830694
OWN - NLM
STAT- MEDLINE
DCOM- 20150630
LR  - 20211021
IS  - 1745-7262 (Electronic)
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 16
IP  - 5
DP  - 2014 Sep-Oct
TI  - Enhancement of mouse germ cell-associated genes expression by injection of human 
      umbilical cord mesenchymal stem cells into the testis of chemical-induced 
      azoospermic mice.
PG  - 698-704
LID - 10.4103/1008-682X.129209 [doi]
AB  - Various methods are currently under investigation to preserve fertility in males 
      treated with high-dose chemotherapy and radiation for malignant and nonmalignant 
      disorders. Human umbilical cord mesenchymal stem cells (HUC-MSCs), which possess 
      potent immunosuppressive function and secrete various cytokines and growth 
      factors, have the potential clinical applications. As a potential alternative, we 
      investigate whether injection of HUC-MSCs into the interstitial compartment of 
      the testes to promote spermatogenic regeneration efficiently. HUC-MSCs were 
      isolated from different sources of umbilical cords and injected into the 
      interstitial space of one testis from 10 busulfan-treated mice (saline and HEK293 
      cells injections were performed in a separate set of mice) and the other testis 
      remained uninjected. Three weeks after MSCs injection, Relative quantitative 
      reverse transcription polymerase chain reaction was used to identify the 
      expression of 10 of germ cell associated, which are all related to meiosis, 
      demonstrated higher levels of spermatogenic gene expression (2-8 fold) in 
      HUC-MSCs injected testes compared to the contralateral uninjected testes (five 
      mice). Protein levels for germ cell-specific genes, miwi, vasa and synaptonemal 
      complex protein (Scp3) were also higher in MSC-treated testes compared to 
      injected controls 3 weeks after treatment. However, no different expression was 
      detected in saline water and HEK293 cells injection control group. We have 
      demonstrated HUC-MSCs could affect mouse germ cell-specific genes expression. The 
      results also provide a possibility that the transplanted HUC-MSCs may promote the 
      recovery of spermatogenesis. This study provides further evidence for preclinical 
      therapeutic effects of HUC-MSCs, and explores a new approach to the treatment of 
      azoospermia.
FAU - Yang, Rui-Feng
AU  - Yang RF
FAU - Liu, Tai-Hua
AU  - Liu TH
FAU - Zhao, Kai
AU  - Zhao K
FAU - Xiong, Cheng-Liang
AU  - Xiong CL
AD  - Center of Reproductive Medicine, Family Planning Research Institute, Tongji 
      Medical College, Huazhong University of Science and Technology; Wuhan Tongji 
      Reproductive Medicine Hospital, Wuhan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Argonaute Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Piwil1 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sycp3 protein, mouse)
RN  - EC 3.6.1.- (Ddx4 protein, mouse)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - Argonaute Proteins/genetics
MH  - Azoospermia/chemically induced/*therapy
MH  - Cell Cycle Proteins
MH  - DEAD-box RNA Helicases/genetics
MH  - DNA-Binding Proteins
MH  - Gene Expression
MH  - HEK293 Cells/transplantation
MH  - Humans
MH  - Injections
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mice
MH  - Nuclear Proteins/genetics
MH  - RNA, Messenger/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spermatogenesis/*genetics
MH  - Spermatozoa/metabolism
MH  - *Testis
MH  - Wharton Jelly/cytology
PMC - PMC4215652
EDAT- 2014/05/17 06:00
MHDA- 2015/07/01 06:00
PMCR- 2014/09/01
CRDT- 2014/05/17 06:00
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2015/07/01 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - 129209 [pii]
AID - AJA-16-698 [pii]
AID - 10.4103/1008-682X.129209 [doi]
PST - ppublish
SO  - Asian J Androl. 2014 Sep-Oct;16(5):698-704. doi: 10.4103/1008-682X.129209.