PMID- 24832038
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20211021
IS  - 1867-0342 (Electronic)
IS  - 1867-0334 (Linking)
VI  - 6
IP  - 2
DP  - 2014 Jun
TI  - Semi-direct lysis of swabs and evaluation of their efficiencies to recover human 
      noroviruses GI and GII from surfaces.
PG  - 132-9
LID - 10.1007/s12560-014-9144-6 [doi]
AB  - Enteric viruses such as noroviruses (NoVs) continue to be the cause of widespread 
      viral outbreaks due to person-to-person transmission, contaminated food, and 
      contaminated surfaces. In order to optimize swabbing methodology for the 
      detection of viruses on (food) contact surfaces, three swab elution/extraction 
      strategies were compared in part one of this study, out of which, one strategy 
      was based on the recently launched ISO protocol (ISO/TS 15216-1) for the 
      determination of hepatitis A virus and NoV in food using real-time RT-PCR 
      (RT-qPCR). These three swab elution/extraction strategies were tested for the 
      detection of GI.4 and GII.4 NoV on high-density polyethylene (HD-PE) surfaces 
      with the use of cotton swabs. For detection of GI.4 and GII.4, the sample 
      recovery efficiency (SRE) obtained with the direct lysis strategy (based on 
      ISO/TS 15216-1) was significantly lower than the SRE obtained with both other 
      strategies. The semi-direct lysis strategy was chosen to assess the SRE of two 
      common swabs (cotton swab and polyester swab) versus the biowipe (Biomerieux, 
      Lyon, France) on three surfaces (HD-PE, neoprene rubber (NR), and nitrile gloves 
      (GL)). For both surfaces, HD-PE and GL, no significant differences in SREs of 
      GI.4 and GII.4 NoVs were detected between the three different swabs. For the 
      coarser NR, biowipes turned out to be the best option for detecting both GI.4 and 
      GII.4 NoV.
FAU - De Keuckelaere, Ann
AU  - De Keuckelaere A
AD  - Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience 
      Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium, 
      Ann.dekeuckelaere@UGent.be.
FAU - Stals, Ambroos
AU  - Stals A
FAU - Uyttendaele, Mieke
AU  - Uyttendaele M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - United States
TA  - Food Environ Virol
JT  - Food and environmental virology
JID - 101483831
SB  - IM
MH  - *Equipment Contamination
MH  - Food/*virology
MH  - Food Handling/*instrumentation
MH  - Food Microbiology/instrumentation/*methods
MH  - Humans
MH  - Norovirus/genetics/*isolation & purification
EDAT- 2014/05/17 06:00
MHDA- 2015/03/31 06:00
CRDT- 2014/05/17 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2014/04/25 00:00 [accepted]
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 10.1007/s12560-014-9144-6 [doi]
PST - ppublish
SO  - Food Environ Virol. 2014 Jun;6(2):132-9. doi: 10.1007/s12560-014-9144-6. Epub 
      2014 May 15.