PMID- 24832111
OWN - NLM
STAT- MEDLINE
DCOM- 20160509
LR  - 20220409
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 154
IP  - 3
DP  - 2014 Jul 3
TI  - Gnetin H isolated from Paeonia anomala inhibits FcepsilonRI-mediated mast cell 
      signaling and degranulation.
PG  - 798-806
LID - S0378-8741(14)00356-0 [pii]
LID - 10.1016/j.jep.2014.05.005 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Paeonia anomala L. is used in Mongolian 
      traditional medicine to treat various diseases including indigestion, abdominal 
      pain, kidney disorders, inflammation, and female diseases. In this study we 
      examined the effects of Paeonia anomala extract (PAE) and compounds derived from 
      Paeonia anomala on immunoglobulin E (IgE)-mediated type I hypersensitivity 
      responses in vitro. MATERIALS AND METHODS: Degranulation assay, reverse 
      transcription PCR, enzyme-lined immunosorbent assays, western blot analyses were 
      performed to measure allergic and proinflammatory mediators in IgE-stimulated rat 
      basophilic leukemia (RBL)-2H3 mast cells treated with or without PAE or gnetin H. 
      RESULTS: Seventeen compounds were isolated, and beta-hexosaminidase release from 
      IgE-stimulated RBL-2H3 mast cells was measured. Of the seventeen isolated 
      compounds, gnetin H, a resveratrol derivative, significantly inhibited 
      beta-hexosaminidase release from RBL-2H3 cells with an IC50 value of 0.3 muM. 
      Notably, Gnetin H reduced beta-hexosaminidase release at lower concentrations than 
      resveratrol. Furthermore, PAE and gnetin H inhibited histamine secretion, 
      decreased the production and mRNA expression of tumor necrosis factor-alpha and 
      interleukin-4 and suppressed translocation of nuclear factor kappaB. PAE and gnetin H 
      also reduced the expression of cyclooxygenase-2 and production of prostaglandin 
      E2. PAE and gnetin H suppressed the phosphorylation of Syk, protein kinase C 
      (PKC)mu, phospholipase Cgamma, and the mitogen-activated protein kinases, c-Jun 
      N-terminal kinase, p38, and extracellular signal-regulated kinase. CONCLUSIONS: 
      These results suggest that PAE and its active compound gnetin H could be 
      promising therapeutic agents for allergic disorders.
CI  - Copyright (c) 2014 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Myungsuk
AU  - Kim M
AD  - Functional Food Center, Korea Institute of Science and Technology (KIST) 
      Gangneung Institute, Gangwon, Korea.
FAU - Lim, Sue Ji
AU  - Lim SJ
AD  - Functional Food Center, Korea Institute of Science and Technology (KIST) 
      Gangneung Institute, Gangwon, Korea; Department of chemistry, Gangneung-Wonju 
      National University, Gangneung, Gangwon-do 210-702, Korea.
FAU - Oidovsambuu, Sarangerel
AU  - Oidovsambuu S
AD  - Functional Food Center, Korea Institute of Science and Technology (KIST) 
      Gangneung Institute, Gangwon, Korea.
FAU - Nho, Chu Won
AU  - Nho CW
AD  - Functional Food Center, Korea Institute of Science and Technology (KIST) 
      Gangneung Institute, Gangwon, Korea. Electronic address: cwnho@kist.re.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (FCER1A protein, rat)
RN  - 0 (Receptors, IgE)
RN  - 0 (Resorcinols)
RN  - 0 (Stilbenes)
RN  - 2RXU4J2Z19 (gnetin H)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Mast Cells/*drug effects/*metabolism
MH  - Molecular Structure
MH  - Paeonia/*chemistry
MH  - Rats
MH  - Receptors, IgE/*antagonists & inhibitors/metabolism
MH  - Resorcinols/chemistry/isolation & purification/*pharmacology
MH  - Signal Transduction/*drug effects
MH  - Stilbenes/chemistry/isolation & purification/*pharmacology
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - FcepsilonRI signaling
OT  - Gnetin H
OT  - IgE-mediated allergic diseases
OT  - Paeonia anomala
OT  - RBL-2H3
EDAT- 2014/05/17 06:00
MHDA- 2016/05/10 06:00
CRDT- 2014/05/17 06:00
PHST- 2014/03/31 00:00 [received]
PHST- 2014/05/05 00:00 [accepted]
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2016/05/10 06:00 [medline]
AID - S0378-8741(14)00356-0 [pii]
AID - 10.1016/j.jep.2014.05.005 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2014 Jul 3;154(3):798-806. doi: 10.1016/j.jep.2014.05.005. Epub 
      2014 May 14.