PMID- 24832455 OWN - NLM STAT- MEDLINE DCOM- 20150109 LR - 20200930 IS - 1934-6069 (Electronic) IS - 1931-3128 (Linking) VI - 15 IP - 5 DP - 2014 May 14 TI - T cells kill bacteria captured by transinfection from dendritic cells and confer protection in mice. PG - 611-22 LID - S1931-3128(14)00140-1 [pii] LID - 10.1016/j.chom.2014.04.006 [doi] AB - Dendritic cells (DCs) phagocytose, process, and present bacterial antigens to T lymphocytes to trigger adaptive immunity. In vivo, bacteria can also be found inside T lymphocytes. However, T cells are refractory to direct bacterial infection, leaving the mechanisms by which bacteria invade T cells unclear. We show that T cells take up bacteria from infected DCs by the process of transinfection, which requires direct contact between the two cells and is enhanced by antigen recognition. Prior to transfer, bacteria localize to the immunological synapse, an intimate DC/T cell contact structure that activates T cells. Strikingly, T cells efficiently eliminate the transinfecting bacteria within the first hours after infection. Transinfected T cells produced high levels of proinflammatory cytokines and were able to protect mice from bacterial challenge following adoptive transfer. Thus, T lymphocytes can capture and kill bacteria in a manner reminiscent of innate immunity. CI - Copyright (c) 2014 Elsevier Inc. All rights reserved. FAU - Cruz-Adalia, Aranzazu AU - Cruz-Adalia A AD - Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas (CNB-CSIC), Department of Molecular and Cellular Biology, Darwin, 3, 28049 Madrid, Spain; Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. FAU - Ramirez-Santiago, Guillermo AU - Ramirez-Santiago G AD - Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas (CNB-CSIC), Department of Molecular and Cellular Biology, Darwin, 3, 28049 Madrid, Spain; Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. FAU - Calabia-Linares, Carmen AU - Calabia-Linares C AD - Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. FAU - Torres-Torresano, Monica AU - Torres-Torresano M AD - Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas (CNB-CSIC), Department of Molecular and Cellular Biology, Darwin, 3, 28049 Madrid, Spain; Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. FAU - Feo, Lidia AU - Feo L AD - Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. FAU - Galan-Diez, Marta AU - Galan-Diez M AD - Instituto de Investigacion Sanitaria Princesa, Hospital de la Princesa, 28006 Madrid, Spain; Microbiology and Immunology Department, Columbia University Medical Center, New York, NY 10032, USA. FAU - Fernandez-Ruiz, Elena AU - Fernandez-Ruiz E AD - Instituto de Investigacion Sanitaria Princesa, Hospital de la Princesa, 28006 Madrid, Spain. FAU - Pereiro, Eva AU - Pereiro E AD - ALBA Synchrotron Light Source, MISTRAL Beamline-Experiments Division, Cerdanyola del Valles, 08290 Barcelona, Spain. FAU - Guttmann, Peter AU - Guttmann P AD - Helmholtz-Zentrum Berlin fur Materialien und Energie GmbH, Institute Soft Matters and Functional Materials, Electron Storage Ring BESSY II, Albert-Einstein-Strasse 15, 12489 Berlin, Germany. FAU - Chiappi, Michele AU - Chiappi M AD - Department Macromolecular, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin, 3, 28049 Madrid, Spain. FAU - Schneider, Gerd AU - Schneider G AD - Helmholtz-Zentrum Berlin fur Materialien und Energie GmbH, Institute Soft Matters and Functional Materials, Electron Storage Ring BESSY II, Albert-Einstein-Strasse 15, 12489 Berlin, Germany. FAU - Carrascosa, Jose Lopez AU - Carrascosa JL AD - Department Macromolecular, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin, 3, 28049 Madrid, Spain; Instituto Madrileno de Estudios Avanzados en Nanociencia (IMDEA Nanociencia), 28049 Cantoblanco, Madrid, Spain. FAU - Chichon, Francisco Javier AU - Chichon FJ AD - Department Macromolecular, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin, 3, 28049 Madrid, Spain. FAU - Martinez Del Hoyo, Gloria AU - Martinez Del Hoyo G AD - Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fernandez Almagro, 3, 28029 Madrid, Spain. FAU - Sanchez-Madrid, Francisco AU - Sanchez-Madrid F AD - Instituto de Investigacion Sanitaria Princesa, Hospital de la Princesa, 28006 Madrid, Spain. FAU - Veiga, Esteban AU - Veiga E AD - Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas (CNB-CSIC), Department of Molecular and Cellular Biology, Darwin, 3, 28049 Madrid, Spain; Instituto de Investigacion Sanitaria Princesa, Hospital de Santa Cristina, 28009 Madrid, Spain. Electronic address: eveiga@cnb.csic.es. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Cell Host Microbe JT - Cell host & microbe JID - 101302316 RN - 0 (Cytokines) SB - IM MH - Animals MH - Bacterial Infections/*microbiology MH - Cells, Cultured MH - Cytokines/immunology MH - Dendritic Cells/*immunology/microbiology MH - Female MH - Humans MH - Immunity, Innate MH - Listeria monocytogenes/*immunology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Phagocytosis MH - Salmonella enterica/*immunology MH - Staphylococcus aureus/*immunology MH - T-Lymphocytes/*immunology/microbiology EDAT- 2014/05/17 06:00 MHDA- 2015/01/13 06:00 CRDT- 2014/05/17 06:00 PHST- 2013/04/18 00:00 [received] PHST- 2014/01/18 00:00 [revised] PHST- 2014/03/16 00:00 [accepted] PHST- 2014/05/17 06:00 [entrez] PHST- 2014/05/17 06:00 [pubmed] PHST- 2015/01/13 06:00 [medline] AID - S1931-3128(14)00140-1 [pii] AID - 10.1016/j.chom.2014.04.006 [doi] PST - ppublish SO - Cell Host Microbe. 2014 May 14;15(5):611-22. doi: 10.1016/j.chom.2014.04.006.