PMID- 24832628
OWN - NLM
STAT- MEDLINE
DCOM- 20150910
LR  - 20211021
IS  - 1532-4206 (Electronic)
IS  - 0743-5800 (Print)
IS  - 0743-5800 (Linking)
VI  - 40
IP  - 1
DP  - 2015
TI  - 3betaHSD and CYB5A double positive adrenocortical cells during adrenal 
      development/aging.
PG  - 8-13
LID - 10.3109/07435800.2014.895377 [doi]
AB  - Androstenedione is a common precursor of sex steroids produced and secreted in 
      the human adrenal gland and produced by 3beta-hydroxysteroid dehydrogenase (3betaHSD), 
      17beta-hydroxylase/17,20-lyase (CYP17) and cytochrome b5 (CYB5A). 3betaHSD is expressed 
      in the zona glomerulosa (ZG) and fasciculata (ZF), CYP17 in the ZF and zona 
      reticularis (ZR) and CYB5A in the ZR, respectively. We previously demonstrated 
      the presence of cortical parenchymal cells co-expressing 3betaHSD and CYB5A with 
      hybrid features of both ZF and ZR in human adrenal cortex and hypothesized that 
      these cells may play an important role in androstenedione production in human 
      adrenal gland. Age-related morphologic development of these hybrid cells has, 
      however, not been studied. Therefore, in this study, 48 human adrenal specimens 
      from various age groups were retrieved. Double-immunohistochemical analyses were 
      used in order to study the correlation between this hybrid cell type and age. In 
      both male and female adrenal cortex, the means of total adrenocortical area, the 
      area positive for CYB5A and its ratio reached highest peak in the 21-40-year-old 
      (y.o.) group. The greatest overlap between 3betaHSD and CYB5A in both total and 
      relative area was present in the 13-20 y.o. group. For all the markers mentioned 
      above, statistically significant differences were detected among the different 
      age groups examined (p < 0.05). These findings indicated that both area and ratio 
      of 3betaHSD and CYB5A double positive cells, which could represent the hybrid cells 
      of ZF and ZR, are correlated with human adrenal development and could 
      subsequently influence age-related serum androstenedione levels.
FAU - Nakamura, Yasuhiro
AU  - Nakamura Y
AD  - Department of Pathology, Tohoku University Graduate School of Medicine , Sendai , 
      Japan and.
FAU - Fujishima, Fumiyoshi
AU  - Fujishima F
FAU - Hui, Xiao-gang
AU  - Hui XG
FAU - Felizola, Saulo J A
AU  - Felizola SJ
FAU - Shibahara, Yukiko
AU  - Shibahara Y
FAU - Akahira, Jun-ichi
AU  - Akahira J
FAU - McNamara, Keely M
AU  - McNamara KM
FAU - Rainey, William E
AU  - Rainey WE
FAU - Sasano, Hironobu
AU  - Sasano H
LA  - eng
GR  - R01 DK069950/DK/NIDDK NIH HHS/United States
GR  - DK069950/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 0 (CYB5A protein, human)
RN  - 9035-39-6 (Cytochromes b5)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Adolescent
MH  - Adrenal Cortex/growth & development/*metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aging/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Cytochromes b5/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Young Adult
PMC - PMC4242803
MID - NIHMS642822
OTO - NOTNLM
OT  - 3beta-Hydroxysteroid dehydrogenase
OT  - cytochrome b5
OT  - development
OT  - double-immunohistochemistry
OT  - human adrenal gland
COIS- Declaration of Interest None of the authors has conflict of interest.
EDAT- 2014/05/17 06:00
MHDA- 2015/09/12 06:00
PMCR- 2016/01/01
CRDT- 2014/05/17 06:00
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2015/09/12 06:00 [medline]
PHST- 2016/01/01 00:00 [pmc-release]
AID - 10.3109/07435800.2014.895377 [doi]
PST - ppublish
SO  - Endocr Res. 2015;40(1):8-13. doi: 10.3109/07435800.2014.895377. Epub 2014 May 15.