PMID- 24833403
OWN - NLM
STAT- MEDLINE
DCOM- 20150630
LR  - 20211021
IS  - 1557-8674 (Electronic)
IS  - 1096-2964 (Print)
IS  - 1096-2964 (Linking)
VI  - 15
IP  - 5
DP  - 2014 Oct
TI  - Bacterial biofilms on implanted suture material are a cause of surgical site 
      infection.
PG  - 592-600
LID - 10.1089/sur.2013.016 [doi]
AB  - BACKGROUND: Surgical site infection (SSI) has been estimated to occur in up to 5% 
      of all procedures, accounting for up to 0.5% of all hospital costs. Bacterial 
      biofilms residing on implanted foreign bodies have been implicated as 
      contributing or causative factors in a wide variety of infectious scenarios, but 
      little consideration has been given to the potential for implanted, submerged 
      suture material to act as a host for biofilm and thus serve as a nidus of 
      infection. METHODS: We report a series of 15 patients who underwent open 
      Roux-en-Y gastric bypass (with musculofascial closure with permanent, 
      multifilament sutures) who developed longstanding and refractory SSIs in the 
      abdominal wall. Explanted suture material at subsequent exploration was examined 
      for biofilm with confocal laser-scanning microscopy (CLSM) and fluorescence in 
      situ hybridization (FISH). RESULTS: All 15 patients at re-exploration were found 
      to have gross evidence of a "slimy" matrix or dense reactive granulation tissue 
      localized to the implanted sutures. Confocal laser-scanning microscopy revealed 
      abundant biofilm present on all sutures examined; FISH was able to identify the 
      presence of specific pathogens in the biofilm. Complete removal of the foreign 
      bodies (and attendant biofilms) resulted in all cases in cure of the SSI. 
      CONCLUSION: Bacterial biofilms on implanted suture material can manifest as 
      persistent surgical site infections that require complete removal of the 
      underlying foreign body substrata for resolution.
FAU - Kathju, Sandeep
AU  - Kathju S
AD  - 1 Department of Plastic Surgery, University of Pittsburgh Medical Center , 
      Pittsburgh, Pennsylvania.
FAU - Nistico, Laura
AU  - Nistico L
FAU - Tower, Irene
AU  - Tower I
FAU - Lasko, Leslie-Ann
AU  - Lasko LA
FAU - Stoodley, Paul
AU  - Stoodley P
LA  - eng
GR  - DE014780/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - United States
TA  - Surg Infect (Larchmt)
JT  - Surgical infections
JID - 9815642
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Adult
MH  - Anti-Bacterial Agents/therapeutic use
MH  - Bacteria/isolation & purification
MH  - *Biofilms
MH  - Cohort Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Surgical Wound Infection/drug therapy/*microbiology/pathology/surgery
MH  - Sutures/*microbiology
PMC - PMC4195429
EDAT- 2014/05/17 06:00
MHDA- 2015/07/01 06:00
PMCR- 2015/10/01
CRDT- 2014/05/17 06:00
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2015/07/01 06:00 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - 10.1089/sur.2013.016 [pii]
AID - 10.1089/sur.2013.016 [doi]
PST - ppublish
SO  - Surg Infect (Larchmt). 2014 Oct;15(5):592-600. doi: 10.1089/sur.2013.016. Epub 
      2014 May 15.