PMID- 24833706
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20141120
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Linking)
VI  - 307
IP  - 1
DP  - 2014 Jul 1
TI  - Experimental evidence and mathematical modeling of thermal effects on human 
      colonic smooth muscle contractility.
PG  - G77-88
LID - 10.1152/ajpgi.00385.2013 [doi]
AB  - It has been shown, in animal models, that gastrointestinal tract (GIT) motility 
      is influenced by temperature; nevertheless, the basic mechanism governing thermal 
      GIT smooth muscle responses has not been fully investigated. Studies based on 
      physiologically tuned mathematical models have predicted that thermal 
      inhomogeneity may induce an electrochemical destabilization of peristaltic 
      activity. In the present study, the effect of thermal cooling on human colonic 
      muscle strip (HCMS) contractility was studied. HCMSs were obtained from 
      disease-free margins of resected segments for cancer. After removal of the mucosa 
      and serosa layers, strips were mounted in separate chambers. After 30 min, 
      spontaneous contractions developed, which were measured using force displacement 
      transducers. Temperature was changed every hour (37, 34, and 31 degrees C). The effect of 
      cooling was analyzed on mean contractile activity, oscillation amplitude, 
      frequency, and contraction to ACh (10(-5) M). At 37 degrees C, HCMSs developed a stable 
      phasic contraction (~0.02 Hz) with a significant ACh-elicited mean contractile 
      response (31% and 22% compared with baseline in the circular and longitudinal 
      axis, respectively). At a lower bath temperature, higher mean contractile 
      amplitude was observed, and it increased in the presence of ACh (78% and 43% 
      higher than the basal tone in the circular and longitudinal axis, respectively, 
      at 31 degrees C). A simplified thermochemomechanical model was tuned on experimental data 
      characterizing the stress state coupling the intracellular Ca(2+) concentration 
      to tissue temperature. In conclusion, acute thermal cooling affects colonic 
      muscular function. Further studies are needed to establish the exact mechanisms 
      involved to better understand clinical consequences of hypothermia on intestinal 
      contractile activity.
CI  - Copyright (c) 2014 the American Physiological Society.
FAU - Altomare, A
AU  - Altomare A
AD  - Gastroenterology Unit, University Campus Bio-Medico of Rome, Rome, Italy;
FAU - Gizzi, A
AU  - Gizzi A
AD  - Nonlinear Physics and Mathematical Modeling Laboratory, University Campus 
      Bio-Medico of Rome, Rome, Italy; and.
FAU - Guarino, M P L
AU  - Guarino MP
AD  - Gastroenterology Unit, University Campus Bio-Medico of Rome, Rome, Italy;
FAU - Loppini, A
AU  - Loppini A
AD  - Nonlinear Physics and Mathematical Modeling Laboratory, University Campus 
      Bio-Medico of Rome, Rome, Italy; and.
FAU - Cocca, S
AU  - Cocca S
AD  - Gastroenterology Unit, University Campus Bio-Medico of Rome, Rome, Italy;
FAU - Dipaola, M
AU  - Dipaola M
AD  - Nonlinear Physics and Mathematical Modeling Laboratory, University Campus 
      Bio-Medico of Rome, Rome, Italy; and Politecnico di Milano, Milano, Italy; and.
FAU - Alloni, R
AU  - Alloni R
AD  - Surgery Unit, University Campus Bio-Medico of Rome, Rome, Italy;
FAU - Cicala, M
AU  - Cicala M
AD  - Gastroenterology Unit, University Campus Bio-Medico of Rome, Rome, Italy; 
      m.cicala@unicampus.it.
FAU - Filippi, S
AU  - Filippi S
AD  - Nonlinear Physics and Mathematical Modeling Laboratory, University Campus 
      Bio-Medico of Rome, Rome, Italy; and International Center for Relativistic 
      Astrophysics, Pescara, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - N9YNS0M02X (Acetylcholine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Aged
MH  - Calcium/metabolism
MH  - *Cold Temperature
MH  - Cold-Shock Response
MH  - Colon/drug effects/metabolism/*physiology
MH  - Female
MH  - *Gastrointestinal Motility/drug effects
MH  - Humans
MH  - In Vitro Techniques
MH  - Male
MH  - Mechanotransduction, Cellular
MH  - Middle Aged
MH  - *Models, Biological
MH  - *Muscle Contraction/drug effects
MH  - Muscle, Smooth/drug effects/metabolism/*physiology
MH  - Time Factors
OTO - NOTNLM
OT  - human colonic smooth muscle strips
OT  - mathematical model
OT  - postoperative ileus
OT  - thermal cooling
OT  - thermochemomechanical coupling
EDAT- 2014/05/17 06:00
MHDA- 2014/08/30 06:00
CRDT- 2014/05/17 06:00
PHST- 2014/05/17 06:00 [entrez]
PHST- 2014/05/17 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - ajpgi.00385.2013 [pii]
AID - 10.1152/ajpgi.00385.2013 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2014 Jul 1;307(1):G77-88. doi: 
      10.1152/ajpgi.00385.2013. Epub 2014 May 15.