PMID- 2483518
OWN - NLM
STAT- MEDLINE
DCOM- 19900508
LR  - 20131121
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 1
IP  - 4
DP  - 1989
TI  - Flow cytometric analysis of rodent epididymal spermatozoal chromatin condensation 
      and loss of free sulfhydryl groups.
PG  - 283-8
AB  - Flow cytometric measurements were made on acridine orange (AO) and 
      7-diethylamino-3-(4'-maleimidylphenyl)-4-methyl-coumarin (CPM)-stained 
      epididymal- and vas deferens-derived spermatozoal nuclei to follow the course of 
      chromatin condensation and oxidation of free sulfhydryl groups, respectively, 
      during passage through mouse and rat posttesticular reproductive tracts. 
      Alterations of mouse and rat spermatozoal chromatin during transition from a 
      testicular elongated spermatids to epididymal caput spermatozoa resulted in a 
      threefold loss of DNA stainability with AO. Passage of spermatozoa from the caput 
      to corpus epididymis was accompanied by an approximate 15% loss of DNA 
      stainability, which was maintained at that level throughout passage into the vas 
      deferens. AO stainability of epididymal spermatozoal nuclei was generally 
      independent of -SH group stainability. CPM stainability of rat spermatozoal 
      nuclei free -SH groups was 83%, 18%, and 11% of caput spermatozoal values for 
      corpus, cauda epididymis, and vas deferens, respectively. Comparable values for 
      mice were 69%, 20%, and 18%. CPM stainability was relatively homogeneous for 
      these mouse and rat reproductive tract regions, except mouse corpus epididymis 
      spermatozoal nuclei stained very heterogeneously. Rat spermatozoa detained by 
      ligature up to 7 days in the caput, corpus, and cauda epididymi had CPM staining 
      values equal to or below those of normal vas spermatozoa, indicating that 
      disulfide (S-S) bonding is intrinsic to the spermatozoa and is independent of the 
      epididymal environment. These data suggest that chromatin condensation and loss 
      of spermatozoal DNA stainability during passage from the testis to the vas 
      deferens are independent of S-S bonding.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Evenson, D P
AU  - Evenson DP
AD  - Department of Chemistry, South Dakota State University, Brookings 57007.
FAU - Baer, R K
AU  - Baer RK
FAU - Jost, L K
AU  - Jost LK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Chromatin)
RN  - 0 (Coumarins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Sulfhydryl Compounds)
RN  - 76877-33-3 (N-(4-(7-diethylamino-4-methylcoumarin-3-yl)phenyl)maleimide)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Chromatin/*metabolism
MH  - Coumarins
MH  - DNA/metabolism
MH  - Epididymis
MH  - Flow Cytometry/methods
MH  - Fluorescent Dyes
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Spermatozoa/*metabolism
MH  - Staining and Labeling
MH  - Sulfhydryl Compounds/*metabolism
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1002/mrd.1080010409 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 1989;1(4):283-8. doi: 10.1002/mrd.1080010409.