PMID- 24836880
OWN - NLM
STAT- MEDLINE
DCOM- 20140810
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 106
DP  - 2014 Aug
TI  - DNA double-strand breaks in relation to persistent organic pollutants in a 
      fasting seabird.
PG  - 68-75
LID - S0147-6513(14)00160-2 [pii]
LID - 10.1016/j.ecoenv.2014.04.020 [doi]
AB  - Lipophilic persistent organic pollutants (POPs) are released from fat reserves 
      during fasting, causing increased blood concentrations. Thus, POPs represent a 
      potential anthropogenic stressor during fasting periods. We analysed the blood of 
      female common eiders (Somateria mollissima) by using agarose gel electrophoresis 
      and image data analysis to quantify the DNA-fraction, of total DNA, that migrated 
      into the gel (DNA-FTM) as a relative measure of DNA double strand-breaks (DSBs) 
      during the fasting incubation period in the high arctic. In 2008 and in 2009 
      blood samples were obtained for analysis of 9 POPs and DNA-FTM at day 5 of the 
      incubation period, and then in the same individuals at day 20. This unique study 
      design gave us the opportunity to analyse the same individuals throughout two 
      points in time, with low and high stress burdens. During the incubation period 
      the body mass (BM) decreased by 21-24%, whereas the POP levels increased by 
      148-639%. The DNA-FTM increased by 61-67% (being proportional to the increase in 
      DSBs). At day 5, but not day 20, DNA-FTM was positively correlated with most 
      analysed POPs. The increase in DNA-FTM was positively correlated with the 
      decrease in BM (g) during incubation. Thus, we suggest that fasting stress (BM 
      loss) decreases DNA integrity and that stress caused by fasting on BM loss 
      appeared to override the additional stress caused by concurrent increase in 
      levels of the analysed POPs in the eiders. Blood levels of POPs in the eiders in 
      Svalbard were relatively low, and additive and/or synergistic genotoxic effects 
      of fasting stress and POP exposure may occur in populations with higher POP 
      levels.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Fenstad, Anette A
AU  - Fenstad AA
AD  - Department of Biology, Norwegian University of Science and Technology, 
      Realfagbygget, 7491 Trondheim, Norway. Electronic address: anettefe@ntnu.no.
FAU - Jenssen, Bjorn M
AU  - Jenssen BM
AD  - Department of Biology, Norwegian University of Science and Technology, 
      Realfagbygget, 7491 Trondheim, Norway. Electronic address: 
      bjorn.munro.jenssen@ntnu.no.
FAU - Moe, Borge
AU  - Moe B
AD  - Norwegian Institute for Nature Research, Framsenteret, 9296 Tromso, Norway. 
      Electronic address: borge.moe@nina.no.
FAU - Hanssen, Sveinn A
AU  - Hanssen SA
AD  - Norwegian Institute for Nature Research, Framsenteret, 9296 Tromso, Norway. 
      Electronic address: sveinn.a.hanssen@nina.no.
FAU - Bingham, Chris
AU  - Bingham C
AD  - Department of Biology, Norwegian University of Science and Technology, 
      Realfagbygget, 7491 Trondheim, Norway. Electronic address: 
      chrisgbingham@gmail.com.
FAU - Herzke, Dorte
AU  - Herzke D
AD  - Norwegian Institute for Air Research, Framsenteret, 9296 Tromso, Norway. 
      Electronic address: dorte.herzke@nilu.no.
FAU - Bustnes, Jan O
AU  - Bustnes JO
AD  - Norwegian Institute for Nature Research, Framsenteret, 9296 Tromso, Norway. 
      Electronic address: Jan.Bustnes@nina.no.
FAU - Krokje, Ase
AU  - Krokje A
AD  - Department of Biology, Norwegian University of Science and Technology, 
      Realfagbygget, 7491 Trondheim, Norway. Electronic address: ase.krokje@ntnu.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140514
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Environmental Pollutants)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adipose Tissue/chemistry/metabolism
MH  - Animals
MH  - *Anseriformes/blood/genetics/metabolism
MH  - Arctic Regions
MH  - DNA/drug effects
MH  - DNA Breaks/*drug effects
MH  - Environmental Pollutants/blood/metabolism/*toxicity
MH  - *Fasting/blood
MH  - Female
MH  - Reproduction/drug effects
MH  - Stress, Physiological/drug effects
OTO - NOTNLM
OT  - Anthropogenic contaminants
OT  - Arctic
OT  - Common eiders
OT  - Genotoxicity
EDAT- 2014/05/20 06:00
MHDA- 2014/08/12 06:00
CRDT- 2014/05/20 06:00
PHST- 2013/10/14 00:00 [received]
PHST- 2014/04/10 00:00 [revised]
PHST- 2014/04/20 00:00 [accepted]
PHST- 2014/05/20 06:00 [entrez]
PHST- 2014/05/20 06:00 [pubmed]
PHST- 2014/08/12 06:00 [medline]
AID - S0147-6513(14)00160-2 [pii]
AID - 10.1016/j.ecoenv.2014.04.020 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2014 Aug;106:68-75. doi: 10.1016/j.ecoenv.2014.04.020. 
      Epub 2014 May 14.