PMID- 24838887
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20140519
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1151
DP  - 2014
TI  - 3D-fluorescence in situ hybridization of intact, anaerobic biofilm.
PG  - 189-97
LID - 10.1007/978-1-4939-0554-6_13 [doi]
AB  - FISH (fluorescence in situ hybridization) is a valuable technique to visualize 
      and quantify localization of different microbial species within biofilms. Biofilm 
      conformation can be altered during typical sample preparation for FISH, which can 
      impact observations in multispecies biofilms, including the relative positions of 
      cells. Here, we describe methods to preserve 3-D structure during FISH for 
      visualization of an anaerobic coculture biofilm of Desulfovibrio vulgaris 
      Hildenborough and Methanococcus maripaludis.
FAU - Brileya, Kristen A
AU  - Brileya KA
AD  - Department of Microbiology, Center for Biofilm Engineering, Montana State 
      University, 173520, 109 Lewis Hall, Bozeman, MT, USA.
FAU - Camilleri, Laura B
AU  - Camilleri LB
FAU - Fields, Matthew W
AU  - Fields MW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Biofilms/growth & development
MH  - Coculture Techniques/methods
MH  - Desulfovibrio vulgaris/cytology/*physiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Methanococcus/cytology/*physiology
EDAT- 2014/05/20 06:00
MHDA- 2015/01/06 06:00
CRDT- 2014/05/20 06:00
PHST- 2014/05/20 06:00 [entrez]
PHST- 2014/05/20 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
AID - 10.1007/978-1-4939-0554-6_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1151:189-97. doi: 10.1007/978-1-4939-0554-6_13.