PMID- 24839168 OWN - NLM STAT- MEDLINE DCOM- 20151015 LR - 20211021 IS - 1559-1174 (Electronic) IS - 1535-1084 (Linking) VI - 16 IP - 3 DP - 2014 Sep TI - MicroRNA expression profile and functional analysis reveal that miR-206 is a critical novel gene for the expression of BDNF induced by ketamine. PG - 594-605 LID - 10.1007/s12017-014-8312-z [doi] AB - Depression is a major social and health concern, and ketamine exerts a quick, remarkable and persistent anti-depressive effect. microRNAs (miRNAs) show remarkable potential in the treatment of clinical depression. Here, we determined the expression profile of miRNAs in the hippocampus of rats treated with ketamine (15 mg/kg). The results suggest that multiple miRNAs were aberrantly expressed in rat hippocampus after ketamine injection (18 miRNAs were significantly reduced, while 22 miRNAs were significantly increased). Among them, miR-206 was down-regulated in ketamine-treated rats. In both cultured neuronal cells in vitro and hippocampus in vivo, we identified that the brain-derived neurotrophic factor (BDNF) was a direct target gene of miR-206. Via this target gene, miR-206 strongly modulated the expression of BDNF. Moreover, overexpression of miR-206 significantly attenuated ketamine-induced up-regulation of BDNF. The results indicated that miRNA-206 was involved in novel therapeutic targets for the anti-depressive effect of ketamine. FAU - Yang, Xiaolin AU - Yang X AD - Department of Anesthesiology, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan Province, China. FAU - Yang, Qinyan AU - Yang Q FAU - Wang, Xiaobin AU - Wang X FAU - Luo, Chunqiong AU - Luo C FAU - Wan, Yunqiang AU - Wan Y FAU - Li, Jiali AU - Li J FAU - Liu, Kezhi AU - Liu K FAU - Zhou, Min AU - Zhou M FAU - Zhang, Chunxiang AU - Zhang C LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140518 PL - United States TA - Neuromolecular Med JT - Neuromolecular medicine JID - 101135365 RN - 0 (Anesthetics, Dissociative) RN - 0 (Antidepressive Agents, Second-Generation) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Excitatory Amino Acid Antagonists) RN - 0 (MicroRNAs) RN - 0 (Oligodeoxyribonucleotides) RN - 0 (mirn206 microRNA, rat) RN - 690G0D6V8H (Ketamine) SB - IM MH - Anesthetics, Dissociative/pharmacology MH - Animals MH - Antidepressive Agents, Second-Generation/*pharmacology MH - Apoptosis/drug effects MH - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics MH - Cells, Cultured MH - Excitatory Amino Acid Antagonists/pharmacology MH - Gene Expression Profiling MH - Gene Expression Regulation/drug effects MH - Gene Knockdown Techniques MH - Hippocampus/cytology/*drug effects/metabolism MH - Ketamine/*pharmacology MH - Male MH - MicroRNAs/biosynthesis/genetics/*physiology MH - Neurons/drug effects/metabolism MH - Oligodeoxyribonucleotides/pharmacology MH - Random Allocation MH - Rats MH - Rats, Sprague-Dawley MH - Transfection EDAT- 2014/05/20 06:00 MHDA- 2015/10/16 06:00 CRDT- 2014/05/20 06:00 PHST- 2014/02/19 00:00 [received] PHST- 2014/05/06 00:00 [accepted] PHST- 2014/05/20 06:00 [entrez] PHST- 2014/05/20 06:00 [pubmed] PHST- 2015/10/16 06:00 [medline] AID - 10.1007/s12017-014-8312-z [doi] PST - ppublish SO - Neuromolecular Med. 2014 Sep;16(3):594-605. doi: 10.1007/s12017-014-8312-z. Epub 2014 May 18.