PMID- 24840404
OWN - NLM
STAT- MEDLINE
DCOM- 20150114
LR  - 20151119
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 32
IP  - 1
DP  - 2014 Jul
TI  - Gastric cancer cell adhesion to laminin enhances acquired chemotherapeutic drug 
      resistance mediated by MGr1-Ag/37LRP.
PG  - 105-14
LID - 10.3892/or.2014.3184 [doi]
AB  - Adhesion of cancer cells to the extracellular matrix (ECM) causes a novel 
      acquired chemotherapeutic drug‑resistant phenotype, referred to as cell 
      adhesion-mediated drug resistance (CAM-DR). Our previous studies suggested that 
      the adhesion molecule MGr1-Ag/37LRP may promote multidrug resistance in gastric 
      cancer cells. Therefore, we investigated MGr1-Ag/37LRP binding-induced adhesion, 
      and its role in CAM-DR. Initial studies revealed that, after adhesion to the ECM, 
      the multidrug-resistant gastric cancer cell lines SGC7901/VCR and SGC7901/ADR 
      showed significantly higher mean adhesive cell numbers than non‑resistant SGC7901 
      cells. We then investigated expression of MGr1-Ag/37LRP in gastric cancer cells 
      adhering to laminin. Western blotting, RT-PCR and dual-luciferase reporter assays 
      showed that laminin induced MGr1-Ag/37LRP expression and activity. In vitro and 
      in vivo assays revealed that small interfering RNA against MGr1-Ag/37LRP 
      significantly reduced CAM-DR in SGC7901/VCR cells. In vivo and in vitro analyses 
      revealed that binding of MGr1-Ag/37LRP decreased intracellular drug accumulation 
      by increasing P-glycoprotein and multidrug-associated protein expression, and 
      inhibited drug-induced apoptosis by regulating Bcl-2 and Bax expression. These 
      results indicate that MGr1-Ag/37LRP contributes to laminin-mediated CAM-DR in 
      gastric cancer cells, and is a potentially effective target for reversing this 
      phenomenon in gastric cancer.
FAU - Sun, Li
AU  - Sun L
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Liu, Lili
AU  - Liu L
AD  - Department of Oncology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an 710038, P.R. China.
FAU - Liu, Xiangqiang
AU  - Liu X
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Wang, Yafang
AU  - Wang Y
AD  - Department of Oncology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an 710038, P.R. China.
FAU - Li, Mengbin
AU  - Li M
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Yao, Liping
AU  - Yao L
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Yang, Jianjun
AU  - Yang J
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Ji, Genlin
AU  - Ji G
AD  - Department of Anesthesiology, Xijing Hospital, Fourth Military Medical 
      University, Xi'an 710032, P.R. China.
FAU - Guo, Changcun
AU  - Guo C
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Pan, Yanglin
AU  - Pan Y
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Liang, Shuhui
AU  - Liang S
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Wang, Biaoluo
AU  - Wang B
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Ding, Jie
AU  - Ding J
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Zhang, Hongwei
AU  - Zhang H
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
FAU - Shi, Yongquan
AU  - Shi Y
AD  - State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, 
      Fourth Military Medical University, Xi'an 710032, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140515
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Laminin)
RN  - 0 (MGr1-antigen, human)
RN  - 5J49Q6B70F (Vincristine)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/*genetics/*metabolism
MH  - Antineoplastic Agents, Phytogenic/therapeutic use
MH  - Apoptosis/drug effects
MH  - Cell Adhesion/drug effects
MH  - Cell Line, Tumor
MH  - Drug Resistance, Neoplasm/*drug effects
MH  - Extracellular Matrix/*metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Laminin/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasms, Experimental
MH  - Stomach Neoplasms/drug therapy/genetics/*pathology
MH  - Vincristine/therapeutic use
EDAT- 2014/05/21 06:00
MHDA- 2015/01/15 06:00
CRDT- 2014/05/21 06:00
PHST- 2014/02/10 00:00 [received]
PHST- 2014/03/22 00:00 [accepted]
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2015/01/15 06:00 [medline]
AID - 10.3892/or.2014.3184 [doi]
PST - ppublish
SO  - Oncol Rep. 2014 Jul;32(1):105-14. doi: 10.3892/or.2014.3184. Epub 2014 May 15.