PMID- 24840468
OWN - NLM
STAT- MEDLINE
DCOM- 20150512
LR  - 20140520
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 125
DP  - 2014 Jul
TI  - Automated and sensitive determination of four anabolic androgenic steroids in 
      urine by online turbulent flow solid-phase extraction coupled with liquid 
      chromatography-tandem mass spectrometry: a novel approach for clinical monitoring 
      and doping control.
PG  - 432-8
LID - S0039-9140(14)00191-X [pii]
LID - 10.1016/j.talanta.2014.03.010 [doi]
AB  - A novel method for automated and sensitive analysis of testosterone, 
      androstenedione, methyltestosterone and methenolone in urine samples by online 
      turbulent flow solid-phase extraction coupled with high performance liquid 
      chromatography-tandem mass spectrometry was developed. The optimization and 
      validation of the method were discussed in detail. The Turboflow C18-P SPE column 
      showed the best extraction efficiency for all the analytes. Nanogram per liter 
      (ng/L) level of AAS could be determined directly and the limits of quantification 
      (LOQs) were 0.01 ng/mL, which were much lower than normally concerned 
      concentrations for these typical anabolic androgenic steroids (AAS) (0.1 ng/mL). 
      The linearity range was from the LOQ to 100 ng/mL for each compound, with the 
      coefficients of determination (r(2)) ranging from 0.9990 to 0.9999. The intraday 
      and interday relative standard deviations (RSDs) ranged from 1.1% to 14.5% (n=5). 
      The proposed method was successfully applied to the analysis of urine samples 
      collected from 24 male athletes and 15 patients of prostate cancer. The proposed 
      method provides an alternative practical way to rapidly determine AAS in urine 
      samples, especially for clinical monitoring and doping control.
CI  - Copyright (c) 2014 Elsevier B.V. All rights reserved.
FAU - Guo, Feng
AU  - Guo F
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Shao, Jing
AU  - Shao J
AD  - National Institute of Sports Medicine, Beijing 100029, China.
FAU - Liu, Qian
AU  - Liu Q
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Shi, Jian-Bo
AU  - Shi JB
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China. Electronic address: jbshi@rcees.ac.cn.
FAU - Jiang, Gui-Bin
AU  - Jiang GB
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140314
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Anabolic Agents)
RN  - 0 (Steroids)
SB  - IM
MH  - Anabolic Agents/*urine
MH  - Automation
MH  - Chromatography, High Pressure Liquid
MH  - *Chromatography, Liquid
MH  - Doping in Sports
MH  - Equipment Design
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Mass Spectrometry
MH  - Prostatic Neoplasms/urine
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Solid Phase Extraction
MH  - Steroids/*urine
MH  - Substance Abuse Detection/*methods
MH  - *Tandem Mass Spectrometry
MH  - Urinalysis/methods
OTO - NOTNLM
OT  - Anabolic androgenic steroids
OT  - Clinical monitoring
OT  - Doping control
OT  - Online turboflow solid-phase extraction
OT  - Urine
EDAT- 2014/05/21 06:00
MHDA- 2015/05/13 06:00
CRDT- 2014/05/21 06:00
PHST- 2013/12/30 00:00 [received]
PHST- 2014/03/02 00:00 [revised]
PHST- 2014/03/10 00:00 [accepted]
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2015/05/13 06:00 [medline]
AID - S0039-9140(14)00191-X [pii]
AID - 10.1016/j.talanta.2014.03.010 [doi]
PST - ppublish
SO  - Talanta. 2014 Jul;125:432-8. doi: 10.1016/j.talanta.2014.03.010. Epub 2014 Mar 
      14.