PMID- 24840765
OWN - NLM
STAT- MEDLINE
DCOM- 20151110
LR  - 20211021
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 8
IP  - 6
DP  - 2014 Jun 24
TI  - Digital sensing and sizing of vesicular stomatitis virus pseudotypes in complex 
      media: a model for Ebola and Marburg detection.
PG  - 6047-6055
LID - 10.1021/nn501312q [doi]
AB  - Rapid, sensitive, and direct label-free capture and characterization of 
      nanoparticles from complex media such as blood or serum will broadly impact 
      medicine and the life sciences. We demonstrate identification of virus particles 
      in complex samples for replication-competent wild-type vesicular stomatitis virus 
      (VSV), defective VSV, and Ebola- and Marburg-pseudotyped VSV with high 
      sensitivity and specificity. Size discrimination of the imaged nanoparticles 
      (virions) allows differentiation between modified viruses having different genome 
      lengths and facilitates a reduction in the counting of nonspecifically bound 
      particles to achieve a limit-of-detection (LOD) of 5 x 10(3) pfu/mL for the Ebola 
      and Marburg VSV pseudotypes. We demonstrate the simultaneous detection of 
      multiple viruses in a single sample (composed of serum or whole blood) for 
      screening applications and uncompromised detection capabilities in samples 
      contaminated with high levels of bacteria. By employing affinity-based capture, 
      size discrimination, and a "digital" detection scheme to count single virus 
      particles, we show that a robust and sensitive virus/nanoparticle sensing assay 
      can be established for targets in complex samples. The nanoparticle microscopy 
      system is termed the Single Particle Interferometric Reflectance Imaging Sensor 
      (SP-IRIS) and is capable of high-throughput and rapid sizing of large numbers of 
      biological nanoparticles on an antibody microarray for research and diagnostic 
      applications.
FAU - Daaboul, George G
AU  - Daaboul GG
AD  - Electrical & Computer Engineering Dept., Boston University, Boston, MA 02215.
FAU - Lopez, Carlos A
AU  - Lopez CA
AD  - Electrical & Computer Engineering Dept., Boston University, Boston, MA 02215.
FAU - Chinnala, Jyothsna
AU  - Chinnala J
AD  - Electrical & Computer Engineering Dept., Boston University, Boston, MA 02215.
FAU - Goldberg, Bennett B
AU  - Goldberg BB
AD  - Electrical & Computer Engineering Dept., Boston University, Boston, MA 02215.
AD  - Biomedical Engineering Dept., Boston University, Boston, MA 02215.
AD  - Physics Department, Boston University, Boston, MA 02215.
FAU - Connor, John H
AU  - Connor JH
AD  - Department of Microbiology and National Emerging and Infectious Diseases 
      Laboratories, Boston University School of Medicine, Boston, MA 02118.
AD  - Boston University Photonics Center, Boston University, Boston, MA 02215.
FAU - Unlu, M Selim
AU  - Unlu MS
AD  - Electrical & Computer Engineering Dept., Boston University, Boston, MA 02215.
AD  - Biomedical Engineering Dept., Boston University, Boston, MA 02215.
AD  - Physics Department, Boston University, Boston, MA 02215.
LA  - eng
GR  - R01 AI096159/AI/NIAID NIH HHS/United States
GR  - R01AI1096159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140604
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (Glycoproteins)
RN  - 0 (Ligands)
SB  - IM
MH  - Animals
MH  - *Biosensing Techniques
MH  - DNA, Complementary/metabolism
MH  - DNA, Viral/analysis
MH  - Glycoproteins/chemistry
MH  - Hemorrhagic Fever, Ebola/*diagnosis/*virology
MH  - Humans
MH  - Interferometry
MH  - Ligands
MH  - Limit of Detection
MH  - Marburg Virus Disease/*diagnosis/*virology
MH  - Nanoparticles/chemistry
MH  - Nanotechnology/methods
MH  - Normal Distribution
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - *Vesiculovirus
PMC - PMC4466106
MID - NIHMS693515
EDAT- 2014/05/21 06:00
MHDA- 2015/11/11 06:00
PMCR- 2015/06/15
CRDT- 2014/05/21 06:00
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2015/11/11 06:00 [medline]
PHST- 2015/06/15 00:00 [pmc-release]
AID - 10.1021/nn501312q [doi]
PST - ppublish
SO  - ACS Nano. 2014 Jun 24;8(6):6047-6055. doi: 10.1021/nn501312q. Epub 2014 Jun 4.