PMID- 24841082
OWN - NLM
STAT- MEDLINE
DCOM- 20141231
LR  - 20240322
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 5
DP  - 2014
TI  - Sexual differences in cell proliferation in the ventricular zone, cell migration 
      and differentiation in the HVC of juvenile Bengalese finch.
PG  - e97403
LID - 10.1371/journal.pone.0097403 [doi]
LID - e97403
AB  - Song control nuclei have distinct sexual differences and thus are an ideal model 
      to address how brain areas are sexually differentiated. Through a combination of 
      histological analysis and electrical lesions, we first identified the ventricle 
      site for HVC progenitor cells. We then found that there were significant sex 
      differences in the cellular proliferation activity in the ventricular zone of the 
      HVC, the number of migrating cells along the radial cells (positive 
      immunoreactions to vimentin) and differentiation towards neurons. Through 
      co-culturing of male and female slices containing the developing HVC in the same 
      well, we found that the male slices could produce diffusible substances to 
      masculinize the female HVC. By adding estrogen, an estrogen antagonist, 
      brain-derived neurotrophic factor (BDNF) or its antibody into the culture medium, 
      separately or in combination, we found that these diffusible substances may 
      include estrogen and BDNF. Finally, we found that 1) estrogen-induced BDNF 
      upregulation could be detected 48 hr after estrogen treatment and could not be 
      blocked by a vascular endothelial growth factor (VEGF) receptor inhibitor and 2) 
      the amount of VEGF mRNA expressed in the developing HVC and its adjacent area did 
      not display any significant sex differences, as did the distribution of VEGF and 
      laminin-expressing endothelial cells in the developing HVC. Because these 
      findings are largely different from previous reports on the adult female HVC, it 
      is suggested that our estrogen-induced BDNF up-regulation and the resultant 
      sexual differentiation might not be mediated by VEGF and endothelial cells, but 
      instead, may result from the direct effects of estrogen on BDNF.
FAU - Chen, Qiong
AU  - Chen Q
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China; Department of Laboratory Medicine, The Third 
      Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
FAU - Zhang, Xuebo
AU  - Zhang X
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China; College of Life Sciences, Hainan Normal University, 
      Haikou, China.
FAU - Zhao, Yueliu
AU  - Zhao Y
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China.
FAU - Sun, Lina
AU  - Sun L
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China.
FAU - Zeng, Shaoju
AU  - Zeng S
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China.
FAU - Zuo, Mingxue
AU  - Zuo M
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, Beijing Normal 
      University, Beijing, China.
FAU - Zhang, Xinwen
AU  - Zhang X
AD  - College of Life Sciences, Hainan Normal University, Haikou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140519
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Cell Movement/*physiology
MH  - Cell Proliferation/*physiology
MH  - Female
MH  - Finches
MH  - High Vocal Center/*cytology
MH  - Male
MH  - Sex Characteristics
PMC - PMC4026142
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/05/21 06:00
MHDA- 2015/01/01 06:00
PMCR- 2014/05/19
CRDT- 2014/05/21 06:00
PHST- 2014/01/18 00:00 [received]
PHST- 2014/04/17 00:00 [accepted]
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2015/01/01 06:00 [medline]
PHST- 2014/05/19 00:00 [pmc-release]
AID - PONE-D-14-02674 [pii]
AID - 10.1371/journal.pone.0097403 [doi]
PST - epublish
SO  - PLoS One. 2014 May 19;9(5):e97403. doi: 10.1371/journal.pone.0097403. eCollection 
      2014.