PMID- 24842047
OWN - NLM
STAT- MEDLINE
DCOM- 20150901
LR  - 20140829
IS  - 1432-0878 (Electronic)
IS  - 0302-766X (Linking)
VI  - 357
IP  - 3
DP  - 2014 Sep
TI  - Reactive oxygen species play a role in muscle wasting during thyrotoxicosis.
PG  - 803-14
LID - 10.1007/s00441-014-1881-1 [doi]
AB  - The role of reactive oxygen species (ROS) in muscle protein hydrolysis and 
      protein oxidation in thyrotoxicosis has not been explored. This study indicates 
      that ROS play a role in skeletal muscle wasting pathways in thyrotoxicosis. Two 
      experimental groups (rats) were treated for 5 days with either 
      3,3',5-triiodothyronine (HT) or HT with alpha-tocopherol (HT + alphaT). Two controls were 
      used, vehicle (Control) and control treated with alphaT (Control + alphaT). Serum T3, 
      peritoneal fat, serum glycerol, muscle and body weight, temperature, 
      mitochondrial metabolism (cytochrome c oxidase activity), oxidative stress 
      parameters and proteolytic activities were examined. High body temperature 
      induced by HT returned to normal when animals were treated with alphaT, although 
      total body and muscle weight did not. An increase in lipolysis was observed in 
      the HT + alphaT group, as peritoneal fat decreased significantly together with an 
      increase in serum glycerol. GSH, GSSG and total radical-trapping antioxidant 
      parameter (TRAP) decreased and catalase activity increased in the HT group. The 
      glutathione redox ratio was higher in HT + alphaT than in both HT and Control + alphaT 
      groups. Carbonyl proteins, AOPP, mitochondrial and chymotrypsin-like proteolytic 
      activities were higher in the HT group than in the Control. HT treatment with alphaT 
      restored mitochondrial metabolism, TRAP, carbonyl protein, chymotrypsin-like 
      activity and AOPP to the level as that of the Control + alphaT. Calpain activity was 
      lower in the HT + alphaT group than in HT and Control + alphaT and superoxide dismutase 
      (SOD) activity was higher in the HT + alphaT group than in the Control + alphaT. Although 
      alphaT did not reverse muscle loss, ROS was involved in proteolysis to some degree.
FAU - Bernardes, Sara Santos
AU  - Bernardes SS
AD  - Laboratory of Molecular Pathology, Department of General Pathology, Universidade 
      Estadual de Londrina, Londrina, Parana State, Brazil.
FAU - Guarnier, Flavia Alessandra
AU  - Guarnier FA
FAU - Marinello, Poliana Camila
AU  - Marinello PC
FAU - Armani, Andre
AU  - Armani A
FAU - Simao, Andrea Name Colado
AU  - Simao AN
FAU - Cecchini, Rubens
AU  - Cecchini R
FAU - Cecchini, Alessandra Lourenco
AU  - Cecchini AL
LA  - eng
PT  - Journal Article
DEP - 20140520
PL  - Germany
TA  - Cell Tissue Res
JT  - Cell and tissue research
JID - 0417625
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 3.4.22.- (Calpain)
RN  - H4N855PNZ1 (alpha-Tocopherol)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Body Weight/drug effects
MH  - Calpain/metabolism
MH  - Cytochromes c/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Muscles/drug effects/*pathology
MH  - Organ Size/drug effects
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Thyrotoxicosis/complications/*pathology
MH  - Triiodothyronine/blood/pharmacology
MH  - Wasting Syndrome/complications/*pathology
MH  - alpha-Tocopherol/pharmacology
EDAT- 2014/05/21 06:00
MHDA- 2015/09/02 06:00
CRDT- 2014/05/21 06:00
PHST- 2013/08/23 00:00 [received]
PHST- 2014/03/27 00:00 [accepted]
PHST- 2014/05/21 06:00 [entrez]
PHST- 2014/05/21 06:00 [pubmed]
PHST- 2015/09/02 06:00 [medline]
AID - 10.1007/s00441-014-1881-1 [doi]
PST - ppublish
SO  - Cell Tissue Res. 2014 Sep;357(3):803-14. doi: 10.1007/s00441-014-1881-1. Epub 
      2014 May 20.